Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages

A comparative study

Prakaimuk Saraithong, Yihong Li, Kanokporn Saenphet, Zhou Chen, Panuwan Chantawannakul

Research output: Contribution to journalArticle

Abstract

Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reaction-denaturing gradient gel electrophoresis analysis (PCR-DGGE) and 16S rRNA sequencing, the aim of this study was to determine the dynamics of bacterial community structure across four A. dorsata life stages in different geographical locations. The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker. However, in the pupal stage, no bands identified as bacteria could be observed. Overall, 2 bacterial phyla (Proteobacteria and Firmicutes) and 4 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacilli) were identified, but the frequency varied among the different stages and locations. The classes of Gammaproteobacteria and Bacilli dominated among larval, newly emerged worker and old worker developmental stages.

Original languageEnglish (US)
JournalInsect Science
DOIs
StateAccepted/In press - 2016

Fingerprint

Apis dorsata
Gammaproteobacteria
honeybee
Bees
gamma-Proteobacteria
Bacilli
developmental stage
bacterial communities
midgut
Bacillus
honey bees
comparative study
Bacteria
developmental stages
Betaproteobacteria
Alphaproteobacteria
Bacterial Structures
Proteobacteria
Denaturing Gradient Gel Electrophoresis
bacterium

Keywords

  • 16S rRNA gene
  • Apis dorsata
  • DGGE
  • Gut flora
  • Insect bacteria
  • Microbial diversity

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages : A comparative study. / Saraithong, Prakaimuk; Li, Yihong; Saenphet, Kanokporn; Chen, Zhou; Chantawannakul, Panuwan.

In: Insect Science, 2016.

Research output: Contribution to journalArticle

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