Microtubule-granule relationships in motile human polymorphonuclear leukocytes

M. I. Ryder, R. N. Weinreb, Richard Niederman

Research output: Contribution to journalArticle

Abstract

We examined the relationship of microtubules to the granule organization in stimulated human polymorphonuclear leukocytes (PMNs). Electron microscopic (EM) observations of critical-point-dried PMNs revealed that only a portion of the granules appeared in close association to microtubules. These closely associated granules appeared to be attached to the microtubule via smaller-diameter filaments. The remaining granules appeared either attached to microtubules at a further distance, via smaller-diameter filaments such as actin, or unassociated with microtubules. EM observations of PMNs treated with either the microtubule promoter drug taxol or the microtubule depolymerization drugs nocodozole and colchicine revealed a redistribution of granules towards the nucleus. Granule clustering at the periphery of the cell was also noted with nocodozole and colchicine. With cytochalasin B, a uniform distribution of granules was noted. However, granule clustering was noted when PMNs were coincubated with cytochalasin B and colchicine. These results indicate that microtubules may have both a direct and indirect role (through other cytoskeletal elements) in the organization of PMN granules.

Original languageEnglish (US)
Pages (from-to)679-686
Number of pages8
JournalAnatomical Record
Volume221
Issue number3
StatePublished - 1988

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Microtubules
microtubules
neutrophils
granules
Neutrophils
drug
electron
Colchicine
colchicine
Cytochalasin B
cytochalasin B
Cluster Analysis
Electrons
electrons
drugs
paclitaxel
depolymerization
Paclitaxel
Pharmaceutical Preparations
Actins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Microtubule-granule relationships in motile human polymorphonuclear leukocytes. / Ryder, M. I.; Weinreb, R. N.; Niederman, Richard.

In: Anatomical Record, Vol. 221, No. 3, 1988, p. 679-686.

Research output: Contribution to journalArticle

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