Microfluidic system for pulsed stimulation and time course analysis of mammalian cells

Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells

Mohammad Qasaimeh, R. Lee, S. Gaudet, D. Juncker

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.

    Original languageEnglish (US)
    Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    PublisherChemical and Biological Microsystems Society
    Pages1075-1077
    Number of pages3
    ISBN (Print)9780979806452
    StatePublished - Jan 1 2012
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
    Duration: Oct 28 2012Nov 1 2012

    Other

    Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    CountryJapan
    CityOkinawa
    Period10/28/1211/1/12

    Fingerprint

    Microfluidics
    Tumor Necrosis Factor-alpha
    Chemical activation
    Cells
    NF-kappa B
    Cell culture

    Keywords

    • HeLa cells
    • Hydrostatic-driven flow
    • Multiple laminar streams
    • Pulsatile stimulation
    • Tumor necrosis factor

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Qasaimeh, M., Lee, R., Gaudet, S., & Juncker, D. (2012). Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1075-1077). Chemical and Biological Microsystems Society.

    Microfluidic system for pulsed stimulation and time course analysis of mammalian cells : Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. / Qasaimeh, Mohammad; Lee, R.; Gaudet, S.; Juncker, D.

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1075-1077.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Qasaimeh, M, Lee, R, Gaudet, S & Juncker, D 2012, Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1075-1077, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
    Qasaimeh M, Lee R, Gaudet S, Juncker D. Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1075-1077
    Qasaimeh, Mohammad ; Lee, R. ; Gaudet, S. ; Juncker, D. / Microfluidic system for pulsed stimulation and time course analysis of mammalian cells : Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1075-1077
    @inproceedings{c842f28a2d8a42b583d646a141f0f671,
    title = "Microfluidic system for pulsed stimulation and time course analysis of mammalian cells: Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells",
    abstract = "We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.",
    keywords = "HeLa cells, Hydrostatic-driven flow, Multiple laminar streams, Pulsatile stimulation, Tumor necrosis factor",
    author = "Mohammad Qasaimeh and R. Lee and S. Gaudet and D. Juncker",
    year = "2012",
    month = "1",
    day = "1",
    language = "English (US)",
    isbn = "9780979806452",
    pages = "1075--1077",
    booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
    publisher = "Chemical and Biological Microsystems Society",

    }

    TY - GEN

    T1 - Microfluidic system for pulsed stimulation and time course analysis of mammalian cells

    T2 - Identification of the minimal TNF-alpha pulse duration for NF-KAPPAB activation in HeLa cells

    AU - Qasaimeh, Mohammad

    AU - Lee, R.

    AU - Gaudet, S.

    AU - Juncker, D.

    PY - 2012/1/1

    Y1 - 2012/1/1

    N2 - We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.

    AB - We present a simple hydrostatic-driven microfluidic system for spatiotemporal cell stimulation. We used the developed system to culture HeLa cancer cells and apply stimulation pulses of Tumor necrosis factor (TNF) as short as 10 s. Using this system, we established the concentration-dependent temporal limit of TNF-stimulated NF-kB activation in HeLa cells, and determined that a 30-seconds pulse is sufficient for full activation of NF-kB with 100 ng/ml TNF, and that a 1-min pulse is needed with 10 ng/ml TNF.

    KW - HeLa cells

    KW - Hydrostatic-driven flow

    KW - Multiple laminar streams

    KW - Pulsatile stimulation

    KW - Tumor necrosis factor

    UR - http://www.scopus.com/inward/record.url?scp=84901760533&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84901760533&partnerID=8YFLogxK

    M3 - Conference contribution

    SN - 9780979806452

    SP - 1075

    EP - 1077

    BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

    PB - Chemical and Biological Microsystems Society

    ER -