Microchip-based enumeration of human white blood cells.

Pierre N. Floriano, Shelley Acosta, Nicolaos Christodoulides, Shannon Weigum, John McDevitt

Research output: Contribution to journalArticle

Abstract

The advent of flow cytometry has considerably changed the ways in which medical testing is conducted. However, the cost of flow cytometers, their large size, and their maintenance needs make them scarce in resource-poor settings and available almost only in clinical pathology laboratories in developed countries. Because cell enumeration is a basic and crucial support of diagnosis, prognosis, and treatment, an alternative cell-counting method that would potentially be cost-effective, portable, and suitable for use in resource-poor settings is warranted. We describe here a protocol for conducting cell-counting experiments in a simple microfluidic structure. This protocol describes how to build a simple microfluidic cell and perform a total white blood cell (WBC) count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.

Original languageEnglish (US)
Pages (from-to)53-64
Number of pages12
JournalMethods in Molecular Biology
Volume385
StatePublished - 2007

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Leukocytes
Microfluidics
Costs and Cost Analysis
Clinical Pathology
Leukocyte Count
Developed Countries
Anti-Idiotypic Antibodies
Flow Cytometry
Maintenance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Floriano, P. N., Acosta, S., Christodoulides, N., Weigum, S., & McDevitt, J. (2007). Microchip-based enumeration of human white blood cells. Methods in Molecular Biology, 385, 53-64.

Microchip-based enumeration of human white blood cells. / Floriano, Pierre N.; Acosta, Shelley; Christodoulides, Nicolaos; Weigum, Shannon; McDevitt, John.

In: Methods in Molecular Biology, Vol. 385, 2007, p. 53-64.

Research output: Contribution to journalArticle

Floriano, PN, Acosta, S, Christodoulides, N, Weigum, S & McDevitt, J 2007, 'Microchip-based enumeration of human white blood cells.', Methods in Molecular Biology, vol. 385, pp. 53-64.
Floriano PN, Acosta S, Christodoulides N, Weigum S, McDevitt J. Microchip-based enumeration of human white blood cells. Methods in Molecular Biology. 2007;385:53-64.
Floriano, Pierre N. ; Acosta, Shelley ; Christodoulides, Nicolaos ; Weigum, Shannon ; McDevitt, John. / Microchip-based enumeration of human white blood cells. In: Methods in Molecular Biology. 2007 ; Vol. 385. pp. 53-64.
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