Microcavity single virus detection and sizing with molecular sensitivity

V. R. Dantham, S. Holler, V. Kolchenko, Z. Wan, S. Arnold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the label-free detection and sizing of the smallest individual RNA virus, MS2 by a spherical microcavity. Mass of this virus is ∼6 ag and produces a theoretical resonance shift ∼0.25 fm upon adsorbing an individual virus at the equator of the bare microcavity, which is well below the r.m.s background noise of 2 fm. However, detection was accomplished with ease (S/N = 8, Q = 4x105) using a single dipole stimulated plasmonic-nanoshell as a microcavity wavelength shift enhancer. Analytical expressions based on the "reactive sensing principle" are developed to extract the radius of the virus from the measured signals. Estimated limit of detection for these experiments was ∼0.4 ag or 240 kDa below the size of all known viruses, largest globular and elongated proteins [Phosphofructokinase (345 kDa) and Fibrinogen (390 kDa), respectively].

Original languageEnglish (US)
Title of host publicationLaser Resonators, Microresonators, and Beam Control XV
Volume8600
DOIs
StatePublished - 2013
EventLaser Resonators, Microresonators, and Beam Control XV - San Francisco, CA, United States
Duration: Feb 3 2013Feb 7 2013

Other

OtherLaser Resonators, Microresonators, and Beam Control XV
CountryUnited States
CitySan Francisco, CA
Period2/3/132/7/13

Fingerprint

Microcavity
Microcavities
sizing
viruses
Viruses
Virus
sensitivity
Nanoshells
Equator
fibrinogen
Phosphofructokinases
shift
Plasmonics
background noise
equators
RNA
Fibrinogen
Dipole
Labels
Sensing

Keywords

  • biosensor
  • localized surface plasmon resonance
  • microcavity
  • virus detection
  • Whispering gallery mode

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Dantham, V. R., Holler, S., Kolchenko, V., Wan, Z., & Arnold, S. (2013). Microcavity single virus detection and sizing with molecular sensitivity. In Laser Resonators, Microresonators, and Beam Control XV (Vol. 8600). [86001P] https://doi.org/10.1117/12.2003098

Microcavity single virus detection and sizing with molecular sensitivity. / Dantham, V. R.; Holler, S.; Kolchenko, V.; Wan, Z.; Arnold, S.

Laser Resonators, Microresonators, and Beam Control XV. Vol. 8600 2013. 86001P.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dantham, VR, Holler, S, Kolchenko, V, Wan, Z & Arnold, S 2013, Microcavity single virus detection and sizing with molecular sensitivity. in Laser Resonators, Microresonators, and Beam Control XV. vol. 8600, 86001P, Laser Resonators, Microresonators, and Beam Control XV, San Francisco, CA, United States, 2/3/13. https://doi.org/10.1117/12.2003098
Dantham VR, Holler S, Kolchenko V, Wan Z, Arnold S. Microcavity single virus detection and sizing with molecular sensitivity. In Laser Resonators, Microresonators, and Beam Control XV. Vol. 8600. 2013. 86001P https://doi.org/10.1117/12.2003098
Dantham, V. R. ; Holler, S. ; Kolchenko, V. ; Wan, Z. ; Arnold, S. / Microcavity single virus detection and sizing with molecular sensitivity. Laser Resonators, Microresonators, and Beam Control XV. Vol. 8600 2013.
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