Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling

Sandra Richardt, Gerrit Timmerhaus, Daniel Lang, Enas Qudeimat, Luiz G.G. Corrêa, Ralf Reski, Stefan A. Rensing, Wolfgang Frank

Research output: Contribution to journalArticle

Abstract

Regulatory networks of salt stress and abscisic acid (ABA) responses have previously been analyzed in seed plants. Here, we report microarray expression profiles of 439 genes encoding transcription-associated proteins (TAPs) in response to salt stress and ABA in the salt-tolerant moss Physcomitrella patens. Fourteen and 56 TAP genes were differentially expressed within 60 min of NaCl and ABA treatment, respectively, indicating that these responses are regulated at the transcriptional level. Overlapping expression profiles, as well as the up-regulation of ABA biosynthesis genes, suggest that ABA mediates the salt stress responses in P. patens. Comparison to public gene expression data of Arabidopsis thaliana and phylogenetic analyses suggest that the role of DREB-like, Dof, and bHLH TAPs in salt stress responses have been conserved during embryophyte evolution, and that the function of ABI3-like, bZIP, HAP3, and CO-like TAPs in seed development and flowering emerged from pre-existing ABA and light signalling pathways.

Original languageEnglish (US)
Pages (from-to)27-45
Number of pages19
JournalPlant Molecular Biology
Volume72
Issue number1-2
DOIs
StatePublished - Jan 1 2010

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Bryopsida
Bryophyta
Physcomitrella patens
Abscisic Acid
Microarray Analysis
salt stress
mosses and liverworts
abscisic acid
Salts
transcription (genetics)
stress response
Seeds
Proteins
proteins
Embryophyta
genes
Carbon Monoxide
Spermatophytina
seed development
acid treatment

Keywords

  • Abscisic acid
  • Gene expression profiling
  • NCED
  • Physcomitrella patens
  • Salt tolerance
  • Transcription factors

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling. / Richardt, Sandra; Timmerhaus, Gerrit; Lang, Daniel; Qudeimat, Enas; Corrêa, Luiz G.G.; Reski, Ralf; Rensing, Stefan A.; Frank, Wolfgang.

In: Plant Molecular Biology, Vol. 72, No. 1-2, 01.01.2010, p. 27-45.

Research output: Contribution to journalArticle

Richardt, Sandra ; Timmerhaus, Gerrit ; Lang, Daniel ; Qudeimat, Enas ; Corrêa, Luiz G.G. ; Reski, Ralf ; Rensing, Stefan A. ; Frank, Wolfgang. / Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling. In: Plant Molecular Biology. 2010 ; Vol. 72, No. 1-2. pp. 27-45.
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