Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling

Sandra Richardt, Gerrit Timmerhaus, Daniel Lang, Enas Qudeimat, Luiz G.G. Corrêa, Ralf Reski, Stefan A. Rensing, Wolfgang Frank

    Research output: Contribution to journalArticle

    Abstract

    Regulatory networks of salt stress and abscisic acid (ABA) responses have previously been analyzed in seed plants. Here, we report microarray expression profiles of 439 genes encoding transcription-associated proteins (TAPs) in response to salt stress and ABA in the salt-tolerant moss Physcomitrella patens. Fourteen and 56 TAP genes were differentially expressed within 60 min of NaCl and ABA treatment, respectively, indicating that these responses are regulated at the transcriptional level. Overlapping expression profiles, as well as the up-regulation of ABA biosynthesis genes, suggest that ABA mediates the salt stress responses in P. patens. Comparison to public gene expression data of Arabidopsis thaliana and phylogenetic analyses suggest that the role of DREB-like, Dof, and bHLH TAPs in salt stress responses have been conserved during embryophyte evolution, and that the function of ABI3-like, bZIP, HAP3, and CO-like TAPs in seed development and flowering emerged from pre-existing ABA and light signalling pathways.

    Original languageEnglish (US)
    Pages (from-to)27-45
    Number of pages19
    JournalPlant Molecular Biology
    Volume72
    Issue number1-2
    DOIs
    StatePublished - Jan 1 2010

    Fingerprint

    Bryopsida
    Bryophyta
    Physcomitrella patens
    Abscisic Acid
    Microarray Analysis
    salt stress
    mosses and liverworts
    abscisic acid
    Salts
    transcription (genetics)
    stress response
    Seeds
    Proteins
    proteins
    Embryophyta
    genes
    Carbon Monoxide
    Spermatophytina
    seed development
    acid treatment

    Keywords

    • Abscisic acid
    • Gene expression profiling
    • NCED
    • Physcomitrella patens
    • Salt tolerance
    • Transcription factors

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Genetics
    • Plant Science

    Cite this

    Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling. / Richardt, Sandra; Timmerhaus, Gerrit; Lang, Daniel; Qudeimat, Enas; Corrêa, Luiz G.G.; Reski, Ralf; Rensing, Stefan A.; Frank, Wolfgang.

    In: Plant Molecular Biology, Vol. 72, No. 1-2, 01.01.2010, p. 27-45.

    Research output: Contribution to journalArticle

    Richardt, Sandra ; Timmerhaus, Gerrit ; Lang, Daniel ; Qudeimat, Enas ; Corrêa, Luiz G.G. ; Reski, Ralf ; Rensing, Stefan A. ; Frank, Wolfgang. / Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling. In: Plant Molecular Biology. 2010 ; Vol. 72, No. 1-2. pp. 27-45.
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