Methods of digital video microscopy for colloidal studies

John C. Crocker, David G. Grier

    Research output: Contribution to journalArticle

    Abstract

    We describe a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions. In a typical application, these direct imaging techniques can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth. Combining information from a sequence of video images into single-particle trajectories makes possible measurements of quantities of fundamental and practical interest such as diffusion coefficients and pair-wise interaction potentials. The measurements we describe in detail combine the outstanding resolution of digital imaging with video-synchronized optical trapping to obtain highly accurate and reproducible results very rapidly.

    Original languageEnglish (US)
    Pages (from-to)298-310
    Number of pages13
    JournalJournal of Colloid and Interface Science
    Volume179
    Issue number1
    DOIs
    StatePublished - Apr 15 1996

    Fingerprint

    Microscopic examination
    microscopy
    Imaging techniques
    particle trajectories
    imaging techniques
    image processing
    colloids
    Suspensions
    Image processing
    Microscopes
    diffusion coefficient
    trapping
    microscopes
    Trajectories
    interactions

    Keywords

    • colloid
    • diffusion coefficient
    • dynamics
    • image processing
    • interaction potential
    • optical tweezers
    • video microscopy

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Methods of digital video microscopy for colloidal studies. / Crocker, John C.; Grier, David G.

    In: Journal of Colloid and Interface Science, Vol. 179, No. 1, 15.04.1996, p. 298-310.

    Research output: Contribution to journalArticle

    Crocker, John C. ; Grier, David G. / Methods of digital video microscopy for colloidal studies. In: Journal of Colloid and Interface Science. 1996 ; Vol. 179, No. 1. pp. 298-310.
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