Metabolic imaging of fatty kidney in diabesity: Validation and dietary intervention

Jacqueline T. Jonker, Paul De Heer, Marten A. Engelse, Evelien H. Van Rossenberg, Celine Q.F. Klessens, Hans J. Baelde, Ingeborg M. Bajema, Sietse Jan Koopmans, Paulo Coelho, Trea C.M. Streefland, Andrew G. Webb, Ilona A. Dekkers, Ton J. Rabelink, Patrick C.N. Rensen, Hildo J. Lamb, Aiko P.J. De Vries

Research output: Contribution to journalArticle

Abstract

Background Obesity and type 2 diabetes have not only been linked to fatty liver, but also to fatty kidney and chronic kidney disease. Since non-invasive tools are lacking to study fatty kidney in clinical studies, we explored agreement between proton magnetic resonance spectroscopy (1 H-MRS) and enzymatic assessment of renal triglyceride content (without and with dietary intervention). We further studied the correlation between fatty kidney and fatty liver. Methods Triglyceride content in the renal cortex was measured by 1 H-MRS on a 7-Tesla scanner in 27 pigs, among which 15 minipigs had been randomized to a 7-month control diet, cafeteria diet (CAF) or CAF with low-dose streptozocin (CAF-S) to induce insulin-independent diabetes. Renal biopsies were taken from corresponding MRS-voxel locations. Additionally, liver biopsies were taken and triglyceride content in all biopsies was measured by enzymatic assay. Results Renal triglyceride content measured by 1 H-MRS and enzymatic assay correlated positively (r = 0.86, P < 0.0001). Compared with control diet-fed minipigs, renal triglyceride content was higher in CAF-S-fed minipigs (137 ± 51 nmol/mg protein, mean ± standard error of the mean, P < 0.05), but not in CAF-fed minipigs (60 ± 10 nmol/mg protein) compared with controls (40 ± 6 nmol/mg protein). Triglyceride contents in liver and kidney biopsies were strongly correlated (r = 0.97, P < 0.001). Conclusions Non-invasive measurement of renal triglyceride content by 1 H-MRS closely predicts triglyceride content as measured enzymatically in biopsies, and fatty kidney appears to develop parallel to fatty liver. 1 H-MRS may be a valuable tool to explore the role of fatty kidney in obesity and type 2 diabetic nephropathy in humans in vivo.

Original languageEnglish (US)
Pages (from-to)224-230
Number of pages7
JournalNephrology Dialysis Transplantation
Volume33
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Kidney
Triglycerides
Miniature Swine
Diet
Biopsy
Fatty Liver
Enzyme Assays
Obesity
Proteins
Liver
Diabetic Nephropathies
Streptozocin
Chronic Renal Insufficiency
Type 2 Diabetes Mellitus
Swine
Insulin

Keywords

  • chronic kidney disease
  • fatty kidney
  • proton magnetic
  • renal triglyceride content
  • resonance spectroscopy
  • type 2 diabetes mellitus

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Jonker, J. T., De Heer, P., Engelse, M. A., Van Rossenberg, E. H., Klessens, C. Q. F., Baelde, H. J., ... De Vries, A. P. J. (2018). Metabolic imaging of fatty kidney in diabesity: Validation and dietary intervention. Nephrology Dialysis Transplantation, 33(2), 224-230. https://doi.org/10.1093/ndt/gfx243

Metabolic imaging of fatty kidney in diabesity : Validation and dietary intervention. / Jonker, Jacqueline T.; De Heer, Paul; Engelse, Marten A.; Van Rossenberg, Evelien H.; Klessens, Celine Q.F.; Baelde, Hans J.; Bajema, Ingeborg M.; Koopmans, Sietse Jan; Coelho, Paulo; Streefland, Trea C.M.; Webb, Andrew G.; Dekkers, Ilona A.; Rabelink, Ton J.; Rensen, Patrick C.N.; Lamb, Hildo J.; De Vries, Aiko P.J.

In: Nephrology Dialysis Transplantation, Vol. 33, No. 2, 01.02.2018, p. 224-230.

Research output: Contribution to journalArticle

Jonker, JT, De Heer, P, Engelse, MA, Van Rossenberg, EH, Klessens, CQF, Baelde, HJ, Bajema, IM, Koopmans, SJ, Coelho, P, Streefland, TCM, Webb, AG, Dekkers, IA, Rabelink, TJ, Rensen, PCN, Lamb, HJ & De Vries, APJ 2018, 'Metabolic imaging of fatty kidney in diabesity: Validation and dietary intervention', Nephrology Dialysis Transplantation, vol. 33, no. 2, pp. 224-230. https://doi.org/10.1093/ndt/gfx243
Jonker JT, De Heer P, Engelse MA, Van Rossenberg EH, Klessens CQF, Baelde HJ et al. Metabolic imaging of fatty kidney in diabesity: Validation and dietary intervention. Nephrology Dialysis Transplantation. 2018 Feb 1;33(2):224-230. https://doi.org/10.1093/ndt/gfx243
Jonker, Jacqueline T. ; De Heer, Paul ; Engelse, Marten A. ; Van Rossenberg, Evelien H. ; Klessens, Celine Q.F. ; Baelde, Hans J. ; Bajema, Ingeborg M. ; Koopmans, Sietse Jan ; Coelho, Paulo ; Streefland, Trea C.M. ; Webb, Andrew G. ; Dekkers, Ilona A. ; Rabelink, Ton J. ; Rensen, Patrick C.N. ; Lamb, Hildo J. ; De Vries, Aiko P.J. / Metabolic imaging of fatty kidney in diabesity : Validation and dietary intervention. In: Nephrology Dialysis Transplantation. 2018 ; Vol. 33, No. 2. pp. 224-230.
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abstract = "Background Obesity and type 2 diabetes have not only been linked to fatty liver, but also to fatty kidney and chronic kidney disease. Since non-invasive tools are lacking to study fatty kidney in clinical studies, we explored agreement between proton magnetic resonance spectroscopy (1 H-MRS) and enzymatic assessment of renal triglyceride content (without and with dietary intervention). We further studied the correlation between fatty kidney and fatty liver. Methods Triglyceride content in the renal cortex was measured by 1 H-MRS on a 7-Tesla scanner in 27 pigs, among which 15 minipigs had been randomized to a 7-month control diet, cafeteria diet (CAF) or CAF with low-dose streptozocin (CAF-S) to induce insulin-independent diabetes. Renal biopsies were taken from corresponding MRS-voxel locations. Additionally, liver biopsies were taken and triglyceride content in all biopsies was measured by enzymatic assay. Results Renal triglyceride content measured by 1 H-MRS and enzymatic assay correlated positively (r = 0.86, P < 0.0001). Compared with control diet-fed minipigs, renal triglyceride content was higher in CAF-S-fed minipigs (137 ± 51 nmol/mg protein, mean ± standard error of the mean, P < 0.05), but not in CAF-fed minipigs (60 ± 10 nmol/mg protein) compared with controls (40 ± 6 nmol/mg protein). Triglyceride contents in liver and kidney biopsies were strongly correlated (r = 0.97, P < 0.001). Conclusions Non-invasive measurement of renal triglyceride content by 1 H-MRS closely predicts triglyceride content as measured enzymatically in biopsies, and fatty kidney appears to develop parallel to fatty liver. 1 H-MRS may be a valuable tool to explore the role of fatty kidney in obesity and type 2 diabetic nephropathy in humans in vivo.",
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T1 - Metabolic imaging of fatty kidney in diabesity

T2 - Validation and dietary intervention

AU - Jonker, Jacqueline T.

AU - De Heer, Paul

AU - Engelse, Marten A.

AU - Van Rossenberg, Evelien H.

AU - Klessens, Celine Q.F.

AU - Baelde, Hans J.

AU - Bajema, Ingeborg M.

AU - Koopmans, Sietse Jan

AU - Coelho, Paulo

AU - Streefland, Trea C.M.

AU - Webb, Andrew G.

AU - Dekkers, Ilona A.

AU - Rabelink, Ton J.

AU - Rensen, Patrick C.N.

AU - Lamb, Hildo J.

AU - De Vries, Aiko P.J.

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N2 - Background Obesity and type 2 diabetes have not only been linked to fatty liver, but also to fatty kidney and chronic kidney disease. Since non-invasive tools are lacking to study fatty kidney in clinical studies, we explored agreement between proton magnetic resonance spectroscopy (1 H-MRS) and enzymatic assessment of renal triglyceride content (without and with dietary intervention). We further studied the correlation between fatty kidney and fatty liver. Methods Triglyceride content in the renal cortex was measured by 1 H-MRS on a 7-Tesla scanner in 27 pigs, among which 15 minipigs had been randomized to a 7-month control diet, cafeteria diet (CAF) or CAF with low-dose streptozocin (CAF-S) to induce insulin-independent diabetes. Renal biopsies were taken from corresponding MRS-voxel locations. Additionally, liver biopsies were taken and triglyceride content in all biopsies was measured by enzymatic assay. Results Renal triglyceride content measured by 1 H-MRS and enzymatic assay correlated positively (r = 0.86, P < 0.0001). Compared with control diet-fed minipigs, renal triglyceride content was higher in CAF-S-fed minipigs (137 ± 51 nmol/mg protein, mean ± standard error of the mean, P < 0.05), but not in CAF-fed minipigs (60 ± 10 nmol/mg protein) compared with controls (40 ± 6 nmol/mg protein). Triglyceride contents in liver and kidney biopsies were strongly correlated (r = 0.97, P < 0.001). Conclusions Non-invasive measurement of renal triglyceride content by 1 H-MRS closely predicts triglyceride content as measured enzymatically in biopsies, and fatty kidney appears to develop parallel to fatty liver. 1 H-MRS may be a valuable tool to explore the role of fatty kidney in obesity and type 2 diabetic nephropathy in humans in vivo.

AB - Background Obesity and type 2 diabetes have not only been linked to fatty liver, but also to fatty kidney and chronic kidney disease. Since non-invasive tools are lacking to study fatty kidney in clinical studies, we explored agreement between proton magnetic resonance spectroscopy (1 H-MRS) and enzymatic assessment of renal triglyceride content (without and with dietary intervention). We further studied the correlation between fatty kidney and fatty liver. Methods Triglyceride content in the renal cortex was measured by 1 H-MRS on a 7-Tesla scanner in 27 pigs, among which 15 minipigs had been randomized to a 7-month control diet, cafeteria diet (CAF) or CAF with low-dose streptozocin (CAF-S) to induce insulin-independent diabetes. Renal biopsies were taken from corresponding MRS-voxel locations. Additionally, liver biopsies were taken and triglyceride content in all biopsies was measured by enzymatic assay. Results Renal triglyceride content measured by 1 H-MRS and enzymatic assay correlated positively (r = 0.86, P < 0.0001). Compared with control diet-fed minipigs, renal triglyceride content was higher in CAF-S-fed minipigs (137 ± 51 nmol/mg protein, mean ± standard error of the mean, P < 0.05), but not in CAF-fed minipigs (60 ± 10 nmol/mg protein) compared with controls (40 ± 6 nmol/mg protein). Triglyceride contents in liver and kidney biopsies were strongly correlated (r = 0.97, P < 0.001). Conclusions Non-invasive measurement of renal triglyceride content by 1 H-MRS closely predicts triglyceride content as measured enzymatically in biopsies, and fatty kidney appears to develop parallel to fatty liver. 1 H-MRS may be a valuable tool to explore the role of fatty kidney in obesity and type 2 diabetic nephropathy in humans in vivo.

KW - chronic kidney disease

KW - fatty kidney

KW - proton magnetic

KW - renal triglyceride content

KW - resonance spectroscopy

KW - type 2 diabetes mellitus

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