Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment

Farah Benyettou, Jaen Alonso Ocadiz Flores, Florent Ravaux, Rachid Rezqui, Mustapha Jouiad, Samer I. Nehme, Rajesh Kumar Parsapur, John Carl Olsen, Parasuraman Selvam, Ali Trabolsi

Research output: Contribution to journalArticle

Abstract

Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50=0.70 μm), wild-type ovarian cancer cells (A2780, IC50=0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50=0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.

Original languageEnglish (US)
Pages (from-to)17020-17028
Number of pages9
JournalChemistry - A European Journal
Volume22
Issue number47
DOIs
StatePublished - Jan 1 2016

Fingerprint

Iron oxides
Doxorubicin
Cells
Nanoparticles
Hybrid systems
Poloxamer
Toxicity
Magnetic fields
Heating
Temperature
Molecules
Carbon
ferric oxide
Polymers
Pharmaceutical Preparations

Keywords

  • controlled release
  • doxorubicin
  • mesoporous iron oxide
  • nanotechnology
  • thermo-chemotherapy

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment. / Benyettou, Farah; Ocadiz Flores, Jaen Alonso; Ravaux, Florent; Rezqui, Rachid; Jouiad, Mustapha; Nehme, Samer I.; Parsapur, Rajesh Kumar; Olsen, John Carl; Selvam, Parasuraman; Trabolsi, Ali.

In: Chemistry - A European Journal, Vol. 22, No. 47, 01.01.2016, p. 17020-17028.

Research output: Contribution to journalArticle

Benyettou, F, Ocadiz Flores, JA, Ravaux, F, Rezqui, R, Jouiad, M, Nehme, SI, Parsapur, RK, Olsen, JC, Selvam, P & Trabolsi, A 2016, 'Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment', Chemistry - A European Journal, vol. 22, no. 47, pp. 17020-17028. https://doi.org/10.1002/chem.201602956
Benyettou, Farah ; Ocadiz Flores, Jaen Alonso ; Ravaux, Florent ; Rezqui, Rachid ; Jouiad, Mustapha ; Nehme, Samer I. ; Parsapur, Rajesh Kumar ; Olsen, John Carl ; Selvam, Parasuraman ; Trabolsi, Ali. / Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment. In: Chemistry - A European Journal. 2016 ; Vol. 22, No. 47. pp. 17020-17028.
@article{00921a20451c49099e991bfd4b066893,
title = "Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment",
abstract = "Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50=0.70 μm), wild-type ovarian cancer cells (A2780, IC50=0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50=0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.",
keywords = "controlled release, doxorubicin, mesoporous iron oxide, nanotechnology, thermo-chemotherapy",
author = "Farah Benyettou and {Ocadiz Flores}, {Jaen Alonso} and Florent Ravaux and Rachid Rezqui and Mustapha Jouiad and Nehme, {Samer I.} and Parsapur, {Rajesh Kumar} and Olsen, {John Carl} and Parasuraman Selvam and Ali Trabolsi",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/chem.201602956",
language = "English (US)",
volume = "22",
pages = "17020--17028",
journal = "Chemistry - A European Journal",
issn = "0947-6539",
publisher = "Wiley-VCH Verlag",
number = "47",

}

TY - JOUR

T1 - Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment

AU - Benyettou, Farah

AU - Ocadiz Flores, Jaen Alonso

AU - Ravaux, Florent

AU - Rezqui, Rachid

AU - Jouiad, Mustapha

AU - Nehme, Samer I.

AU - Parsapur, Rajesh Kumar

AU - Olsen, John Carl

AU - Selvam, Parasuraman

AU - Trabolsi, Ali

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50=0.70 μm), wild-type ovarian cancer cells (A2780, IC50=0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50=0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.

AB - Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50=0.70 μm), wild-type ovarian cancer cells (A2780, IC50=0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50=0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.

KW - controlled release

KW - doxorubicin

KW - mesoporous iron oxide

KW - nanotechnology

KW - thermo-chemotherapy

UR - http://www.scopus.com/inward/record.url?scp=84991448085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991448085&partnerID=8YFLogxK

U2 - 10.1002/chem.201602956

DO - 10.1002/chem.201602956

M3 - Article

C2 - 27739116

AN - SCOPUS:84991448085

VL - 22

SP - 17020

EP - 17028

JO - Chemistry - A European Journal

JF - Chemistry - A European Journal

SN - 0947-6539

IS - 47

ER -