Merging orthogonal microfluidic flows to generate multi-profile concentration gradients

A. Menachery, N. Kumawat, Mohammad Qasaimeh

    Research output: Contribution to journalArticle

    Abstract

    This work describes a novel microfluidic device capable of generating multi-profile gradients that include sigmoidal, parabolic, and exponential concentration variations across its main channel. The main distinguishing feature of this device is its simple geometry: it contains fewer fluidic channels that provide versatility and ease of operation. The narrow orthogonal side channels transport analyte into a wider buffer stream, and by merely altering flow rates of either one or both streams, gradient profiles are switched from one to another. Finite element simulations match well with the experimental results and demonstrate simple manipulation of the generated gradients. Results show that the gradient's slope, extent, and position can be modulated by subtle flow rate variations, making the platform adaptable for various biological applications. The simplicity of the device offers potential for stable chemotactic studies for long durations.

    Original languageEnglish (US)
    Pages (from-to)45513-45520
    Number of pages8
    JournalRSC Advances
    Volume7
    Issue number72
    DOIs
    StatePublished - Jan 1 2017

    Fingerprint

    Merging
    Microfluidics
    Flow rate
    Fluidics
    Buffers
    Geometry

    ASJC Scopus subject areas

    • Chemistry(all)
    • Chemical Engineering(all)

    Cite this

    Merging orthogonal microfluidic flows to generate multi-profile concentration gradients. / Menachery, A.; Kumawat, N.; Qasaimeh, Mohammad.

    In: RSC Advances, Vol. 7, No. 72, 01.01.2017, p. 45513-45520.

    Research output: Contribution to journalArticle

    Menachery, A. ; Kumawat, N. ; Qasaimeh, Mohammad. / Merging orthogonal microfluidic flows to generate multi-profile concentration gradients. In: RSC Advances. 2017 ; Vol. 7, No. 72. pp. 45513-45520.
    @article{6421f36d619f4c97b0a30f8c037ddbf4,
    title = "Merging orthogonal microfluidic flows to generate multi-profile concentration gradients",
    abstract = "This work describes a novel microfluidic device capable of generating multi-profile gradients that include sigmoidal, parabolic, and exponential concentration variations across its main channel. The main distinguishing feature of this device is its simple geometry: it contains fewer fluidic channels that provide versatility and ease of operation. The narrow orthogonal side channels transport analyte into a wider buffer stream, and by merely altering flow rates of either one or both streams, gradient profiles are switched from one to another. Finite element simulations match well with the experimental results and demonstrate simple manipulation of the generated gradients. Results show that the gradient's slope, extent, and position can be modulated by subtle flow rate variations, making the platform adaptable for various biological applications. The simplicity of the device offers potential for stable chemotactic studies for long durations.",
    author = "A. Menachery and N. Kumawat and Mohammad Qasaimeh",
    year = "2017",
    month = "1",
    day = "1",
    doi = "10.1039/c7ra09692e",
    language = "English (US)",
    volume = "7",
    pages = "45513--45520",
    journal = "RSC Advances",
    issn = "2046-2069",
    publisher = "Royal Society of Chemistry",
    number = "72",

    }

    TY - JOUR

    T1 - Merging orthogonal microfluidic flows to generate multi-profile concentration gradients

    AU - Menachery, A.

    AU - Kumawat, N.

    AU - Qasaimeh, Mohammad

    PY - 2017/1/1

    Y1 - 2017/1/1

    N2 - This work describes a novel microfluidic device capable of generating multi-profile gradients that include sigmoidal, parabolic, and exponential concentration variations across its main channel. The main distinguishing feature of this device is its simple geometry: it contains fewer fluidic channels that provide versatility and ease of operation. The narrow orthogonal side channels transport analyte into a wider buffer stream, and by merely altering flow rates of either one or both streams, gradient profiles are switched from one to another. Finite element simulations match well with the experimental results and demonstrate simple manipulation of the generated gradients. Results show that the gradient's slope, extent, and position can be modulated by subtle flow rate variations, making the platform adaptable for various biological applications. The simplicity of the device offers potential for stable chemotactic studies for long durations.

    AB - This work describes a novel microfluidic device capable of generating multi-profile gradients that include sigmoidal, parabolic, and exponential concentration variations across its main channel. The main distinguishing feature of this device is its simple geometry: it contains fewer fluidic channels that provide versatility and ease of operation. The narrow orthogonal side channels transport analyte into a wider buffer stream, and by merely altering flow rates of either one or both streams, gradient profiles are switched from one to another. Finite element simulations match well with the experimental results and demonstrate simple manipulation of the generated gradients. Results show that the gradient's slope, extent, and position can be modulated by subtle flow rate variations, making the platform adaptable for various biological applications. The simplicity of the device offers potential for stable chemotactic studies for long durations.

    UR - http://www.scopus.com/inward/record.url?scp=85030213071&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85030213071&partnerID=8YFLogxK

    U2 - 10.1039/c7ra09692e

    DO - 10.1039/c7ra09692e

    M3 - Article

    VL - 7

    SP - 45513

    EP - 45520

    JO - RSC Advances

    JF - RSC Advances

    SN - 2046-2069

    IS - 72

    ER -