Membrane-based on-line optical analysis system for rapid detection of bacteria and spores

Pierre N. Floriano, Nick Christodoulides, Dwight Romanovicz, Bruce Bernard, Glennon W. Simmons, Myles Cavell, John McDevitt

Research output: Contribution to journalArticle

Abstract

We report here the adaptation of our electronic microchip technology towards the development of a new method for detecting and enumerating bacterial cells and spores. This new approach is based on the immuno-localization of bacterial spores captured on a membrane filter microchip placed within a flow cell. A combination of microfluidic, optical, and software components enables the integration of staining of the bacterial species with fully automated assays. The quantitation of the analyte signal is achieved through the measurement of a collective response or alternatively through the identification and counting of individual spores and particles. This new instrument displays outstanding analytical characteristics, and presents a limit of detection of ∼500 spores when tested with Bacillus globigii (Bg), a commonly used simulant for Bacillus anthracis (Ba), with a total analysis time of only 5 min. Additionally, the system performed well when tested with real postal dust samples spiked with Bg in the presence of other common contaminants. This new approach is highly customizable towards a large number of relevant toxic chemicals, environmental factors, and analytes of relevance to clinical chemistry applications.

Original languageEnglish (US)
Pages (from-to)2079-2088
Number of pages10
JournalBiosensors and Bioelectronics
Volume20
Issue number10 SPEC. ISS.
DOIs
StatePublished - Apr 15 2005

Fingerprint

Bacterial Spores
Optical Devices
Bacilli
Spores
Bacillus
Bacteria
Membranes
Bacillus anthracis
Clinical Chemistry
Microfluidics
Poisons
Instrument displays
Dust
Limit of Detection
Software
Staining and Labeling
Technology
Assays
Impurities

Keywords

  • B. anthracis
  • B. globigii
  • Bacteria
  • Epifluorescence
  • Immunoassay
  • Microchip

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Floriano, P. N., Christodoulides, N., Romanovicz, D., Bernard, B., Simmons, G. W., Cavell, M., & McDevitt, J. (2005). Membrane-based on-line optical analysis system for rapid detection of bacteria and spores. Biosensors and Bioelectronics, 20(10 SPEC. ISS.), 2079-2088. https://doi.org/10.1016/j.bios.2004.08.046

Membrane-based on-line optical analysis system for rapid detection of bacteria and spores. / Floriano, Pierre N.; Christodoulides, Nick; Romanovicz, Dwight; Bernard, Bruce; Simmons, Glennon W.; Cavell, Myles; McDevitt, John.

In: Biosensors and Bioelectronics, Vol. 20, No. 10 SPEC. ISS., 15.04.2005, p. 2079-2088.

Research output: Contribution to journalArticle

Floriano, PN, Christodoulides, N, Romanovicz, D, Bernard, B, Simmons, GW, Cavell, M & McDevitt, J 2005, 'Membrane-based on-line optical analysis system for rapid detection of bacteria and spores', Biosensors and Bioelectronics, vol. 20, no. 10 SPEC. ISS., pp. 2079-2088. https://doi.org/10.1016/j.bios.2004.08.046
Floriano PN, Christodoulides N, Romanovicz D, Bernard B, Simmons GW, Cavell M et al. Membrane-based on-line optical analysis system for rapid detection of bacteria and spores. Biosensors and Bioelectronics. 2005 Apr 15;20(10 SPEC. ISS.):2079-2088. https://doi.org/10.1016/j.bios.2004.08.046
Floriano, Pierre N. ; Christodoulides, Nick ; Romanovicz, Dwight ; Bernard, Bruce ; Simmons, Glennon W. ; Cavell, Myles ; McDevitt, John. / Membrane-based on-line optical analysis system for rapid detection of bacteria and spores. In: Biosensors and Bioelectronics. 2005 ; Vol. 20, No. 10 SPEC. ISS. pp. 2079-2088.
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