MEL-28 Is Downstream of the Ran Cycle and Is Required for Nuclear-Envelope Function and Chromatin Maintenance

Anita G. Fernandez, Fabio Piano

Research output: Contribution to journalArticle

Abstract

Early embryonic development depends on the faithful execution of basic cell biological processes whose coordination remains largely unknown. With a global network analysis, we found MEL-28 to be associated with two types of complexes, one implicated in nuclear-envelope function and the other in chromatin organization [1]. Here, we show that MEL-28, a protein that shuttles between the nucleus and the kinetochore during the cell cycle, is required for the structural and functional integrity of the nuclear envelope. In addition, mel-28(RNAi) embryos exhibit defects in chromosome condensation, pronuclear migration, kinetochore assembly, and spindle assembly. This combination of mel-28(RNAi) phenotypes resemble those caused by depleting members of the Ran cycle in C. elegans [2], a conserved cellular signaling pathway that is required for mitotic spindle assembly, nuclear-envelope reformation after mitosis, and nucleocytoplasmic exchange (reviewed in [3-8]). Although MEL-28 localization to the nuclear periphery is not dependent on nuclear pore components, it is dependent on RAN-1 and other key components of the Ran cycle. Thus, MEL-28 is downstream of the Ran cycle and is required for both proper nuclear-envelope function and chromatin maintenance.

Original languageEnglish (US)
Pages (from-to)1757-1763
Number of pages7
JournalCurrent Biology
Volume16
Issue number17
DOIs
StatePublished - Sep 5 2006

Fingerprint

Nuclear Envelope
nuclear membrane
Chromatin
chromatin
Maintenance
Kinetochores
RNA Interference
kinetochores
Cell signaling
Chromosomes
Electric network analysis
Biological Phenomena
Nuclear Pore
Spindle Apparatus
Condensation
cell communication
Cells
mitotic spindle apparatus
Mitosis
Embryonic Development

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

MEL-28 Is Downstream of the Ran Cycle and Is Required for Nuclear-Envelope Function and Chromatin Maintenance. / Fernandez, Anita G.; Piano, Fabio.

In: Current Biology, Vol. 16, No. 17, 05.09.2006, p. 1757-1763.

Research output: Contribution to journalArticle

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