Medial amygdala lesions selectively block aversive pavlovian-instrumental transfer in rats

Margaret G. McCue, Joseph Ledoux, Christopher K. Cain

Research output: Contribution to journalArticle

Abstract

Pavlovian conditioned stimuli (CSs) play an important role in the reinforcement and motivation of instrumental active avoidance (AA). Conditioned threats can also invigorate ongoing AA responding [aversive Pavlovian-instrumental transfer (PIT)]. The neural circuits mediating AA are poorly understood, although lesion studies suggest that lateral, basal, and central amygdala nuclei, as well as infralimbic prefrontal cortex, make key, and sometimes opposing, contributions. We recently completed an extensive analysis of brain c-Fos expression in good vs. poor avoiders following an AA test (Martinez et al., 2013, Learning and Memory). This analysis identified medial amygdala (MeA) as a potentially important region for Pavlovian motivation of instrumental actions. MeA is known to mediate defensive responding to innate threats as well as social behaviors, but its role in mediating aversive Pavlovian-instrumental interactions is unknown. We evaluated the effect of MeA lesions on Pavlovian conditioning, Sidman two-way AA conditioning (shuttling) and aversive PIT in rats. Mild footshocks served as the unconditioned stimulus in all conditioning phases. MeA lesions had no effect on AA but blocked the expression of aversive PIT and 22 kHz ultrasonic vocalizations in the AA context. Interestingly, MeA lesions failed to affect Pavlovian freezing to discrete threats but reduced freezing to contextual threats when assessed outside of the AA chamber. These findings differentiate MeA from lateral and central amygdala, as lesions of these nuclei disrupt Pavlovian freezing and aversive PIT, but have opposite effects on AA performance. Taken together, these results suggest that MeA plays a selective role in the motivation of instrumental avoidance by general or uncertain Pavlovian threats.

Original languageEnglish (US)
Article number329
JournalFrontiers in Behavioral Neuroscience
Volume8
DOIs
StatePublished - Sep 18 2014

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Amygdala
Freezing
Motivation
Instinct
Social Behavior
Transfer (Psychology)
Prefrontal Cortex
Ultrasonics
Learning
Brain
Conditioning (Psychology)

Keywords

  • Amygdala
  • Avoidance
  • Freezing
  • Instrumental
  • Medial
  • Pavlovian
  • Transfer
  • Ultrasonic

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Cognitive Neuroscience
  • Neuropsychology and Physiological Psychology

Cite this

Medial amygdala lesions selectively block aversive pavlovian-instrumental transfer in rats. / McCue, Margaret G.; Ledoux, Joseph; Cain, Christopher K.

In: Frontiers in Behavioral Neuroscience, Vol. 8, 329, 18.09.2014.

Research output: Contribution to journalArticle

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