Mechanisms of femtosecond laser cell surgery in the low-density plasma regime

K. Kuetemeyer, K. Kuetemeyer, Rachid Rezqui, H. Lubatschowski, A. Heisterkamp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXII
Volume7897
DOIs
StatePublished - Apr 29 2011
EventOptical Interactions with Tissue and Cells XXII - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Other

OtherOptical Interactions with Tissue and Cells XXII
CountryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Fingerprint

Plasma density
Laser Therapy
Ablation
Ultrashort pulses
surgery
Surgery
ablation
plasma density
Electrons
Staining and Labeling
staining
cells
Free Radicals
lasers
Cell Biology
Seeds
Lasers
Coloring Agents
Fluorescence
Cytology

Keywords

  • Cell surgery
  • Femtosecond laser
  • Free radicals
  • Low-density plasma
  • Photochemistry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kuetemeyer, K., Kuetemeyer, K., Rezqui, R., Lubatschowski, H., & Heisterkamp, A. (2011). Mechanisms of femtosecond laser cell surgery in the low-density plasma regime. In Optical Interactions with Tissue and Cells XXII (Vol. 7897). [789704] https://doi.org/10.1117/12.874147

Mechanisms of femtosecond laser cell surgery in the low-density plasma regime. / Kuetemeyer, K.; Kuetemeyer, K.; Rezqui, Rachid; Lubatschowski, H.; Heisterkamp, A.

Optical Interactions with Tissue and Cells XXII. Vol. 7897 2011. 789704.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kuetemeyer, K, Kuetemeyer, K, Rezqui, R, Lubatschowski, H & Heisterkamp, A 2011, Mechanisms of femtosecond laser cell surgery in the low-density plasma regime. in Optical Interactions with Tissue and Cells XXII. vol. 7897, 789704, Optical Interactions with Tissue and Cells XXII, San Francisco, CA, United States, 1/24/11. https://doi.org/10.1117/12.874147
Kuetemeyer K, Kuetemeyer K, Rezqui R, Lubatschowski H, Heisterkamp A. Mechanisms of femtosecond laser cell surgery in the low-density plasma regime. In Optical Interactions with Tissue and Cells XXII. Vol. 7897. 2011. 789704 https://doi.org/10.1117/12.874147
Kuetemeyer, K. ; Kuetemeyer, K. ; Rezqui, Rachid ; Lubatschowski, H. ; Heisterkamp, A. / Mechanisms of femtosecond laser cell surgery in the low-density plasma regime. Optical Interactions with Tissue and Cells XXII. Vol. 7897 2011.
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