Mechanisms of cytokine-mediated inhibition of viral replication

Takashi Komatsu, Neil Srivastava, Margarita Revzin, Derek D C Ireland, David Chesler, Carol Shoshkes Reiss

Research output: Contribution to journalArticle

Abstract

In this report, the role of nitric oxide synthase (NOS) and IL-12 administration in inhibition of vesicular stomatitis virus (VSV) from infected neuroblastoma cells was examined. We previously have shown that cytokine treatment of cells results in the induction of NOS-1, and this is associated with a 2 log inhibition of VSV production. We performed these studies to examine the mechanism by which viral replication is suppressed. Neuroblastoma cells (NB41A3) were treated with either IL-12 or medium and subsequently infected with VSV. Viral protein and mRNA were isolated from these cells, and their levels were measured by Western or Northern blots, respectively, mRNA levels were decreased modestly, but viral proteins were decreased substantially in cells pretreated with IL-12, suggesting that the inhibitory effect of NO is working at the translational level. Cytokine treatment of cells was not associated with oxidative stress. The vital proteins also were nitrosylated. These data suggest that the mechanism of NO inhibition of vital replication occurs through translational interference and posttranslational modifications of viral components.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalVirology
Volume259
Issue number2
DOIs
StatePublished - Jul 5 1999

    Fingerprint

Keywords

  • Acquired immunity
  • Innate immunity
  • Interleukin-12
  • Nitric oxide synthase
  • Viral infection

ASJC Scopus subject areas

  • Virology

Cite this

Komatsu, T., Srivastava, N., Revzin, M., Ireland, D. D. C., Chesler, D., & Shoshkes Reiss, C. (1999). Mechanisms of cytokine-mediated inhibition of viral replication. Virology, 259(2), 334-341. https://doi.org/10.1006/viro.1999.9801