Mechanics regulates fate decisions of human embryonic stem cells

Yubing Sun, Luis G. Villa-Diaz, Raymond H W Lam, Weiqiang Chen, Paul H. Krebsbach, Jianping Fu

Research output: Contribution to journalArticle

Abstract

Research on human embryonic stem cells (hESCs) has attracted much attention given their great potential for tissue regenerative therapy and fundamental developmental biology studies. Yet, there is still limited understanding of how mechanical signals in the local cellular microenvironment of hESCs regulate their fate decisions. Here, we applied a microfabricated micromechanical platform to investigate the mechanoresponsive behaviors of hESCs. We demonstrated that hESCs are mechanosensitive, and they could increase their cytoskeleton contractility with matrix rigidity. Furthermore, rigid substrates supported maintenance of pluripotency of hESCs. Matrix mechanics-mediated cytoskeleton contractility might be functionally correlated with E-cadherin expressions in cell-cell contacts and thus involved in fate decisions of hESCs. Our results highlighted the important functional link between matrix rigidity, cellular mechanics, and pluripotency of hESCs and provided a novel approach to characterize and understand mechanotransduction and its involvement in hESC function.

Original languageEnglish (US)
Article numbere37178
JournalPLoS One
Volume7
Issue number5
DOIs
StatePublished - May 16 2012

Fingerprint

embryonic stem cells
Stem cells
Mechanics
mechanics
cytoskeleton
Cytoskeleton
Rigidity
Cellular Microenvironment
Developmental Biology
Human Embryonic Stem Cells
cadherins
human behavior
Cadherins
Cell- and Tissue-Based Therapy
Maintenance
cells
Tissue
Biological Sciences
therapeutics
Substrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sun, Y., Villa-Diaz, L. G., Lam, R. H. W., Chen, W., Krebsbach, P. H., & Fu, J. (2012). Mechanics regulates fate decisions of human embryonic stem cells. PLoS One, 7(5), [e37178]. https://doi.org/10.1371/journal.pone.0037178

Mechanics regulates fate decisions of human embryonic stem cells. / Sun, Yubing; Villa-Diaz, Luis G.; Lam, Raymond H W; Chen, Weiqiang; Krebsbach, Paul H.; Fu, Jianping.

In: PLoS One, Vol. 7, No. 5, e37178, 16.05.2012.

Research output: Contribution to journalArticle

Sun, Y, Villa-Diaz, LG, Lam, RHW, Chen, W, Krebsbach, PH & Fu, J 2012, 'Mechanics regulates fate decisions of human embryonic stem cells', PLoS One, vol. 7, no. 5, e37178. https://doi.org/10.1371/journal.pone.0037178
Sun, Yubing ; Villa-Diaz, Luis G. ; Lam, Raymond H W ; Chen, Weiqiang ; Krebsbach, Paul H. ; Fu, Jianping. / Mechanics regulates fate decisions of human embryonic stem cells. In: PLoS One. 2012 ; Vol. 7, No. 5.
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