Mechanical regulation of cardiac myofibrillar structure

D. G. Simpson, W. W. Sharp, T. K. Borg, R. L. Price, A. M. Samarel, Louis Terracio

Research output: Contribution to journalArticle

Abstract

The excitation-contraction coupling cycle (ECC) consists of a complex cascade of electrochemical and mechanical events; however, the relative contributions of these different processes in the regulation of cardiac myofibrillar structure are not well understood. There is extensive evidence to suggest that the mechanical aspects of the ECC play a crucial role in controlling the availability of contractile proteins for myofibrillar assembly. To examine if these physical forces might also serve to stabilize the structure of preexisting myofibrils, beating and nonbeating cultures of neonatal cardiac myocytes (NCM) were subjected to a 5% static stretch. Contractile arrest was achieved by treating NCM with 12 μM nifedipine, which resulted in immediate and sustained contractile arrest and initiated the evolution of marked myofibrillar abnormalities within 24 hours. As judged by scanning confocal and transmission electron microscopic examination, an external load appears to partially stabilize myofibrillar structure in nonbeating NCM. These results suggest that the maintenance of myofibrillar structure may be highly dependent upon the mechanical aspects of ECC.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalAnnals of the New York Academy of Sciences
Volume752
DOIs
StatePublished - 1995

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Excitation Contraction Coupling
Cardiac Myocytes
Contractile Proteins
Myofibrils
Nifedipine
Microscopic examination
Maintenance
Availability
Electrons
Scanning
Contraction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mechanical regulation of cardiac myofibrillar structure. / Simpson, D. G.; Sharp, W. W.; Borg, T. K.; Price, R. L.; Samarel, A. M.; Terracio, Louis.

In: Annals of the New York Academy of Sciences, Vol. 752, 1995, p. 131-140.

Research output: Contribution to journalArticle

Simpson, D. G. ; Sharp, W. W. ; Borg, T. K. ; Price, R. L. ; Samarel, A. M. ; Terracio, Louis. / Mechanical regulation of cardiac myofibrillar structure. In: Annals of the New York Academy of Sciences. 1995 ; Vol. 752. pp. 131-140.
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