Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes

Will W. Sharp, David G. Simpson, Thomas K. Borg, Allen M. Samarel, Louis Terracio

Research output: Contribution to journalArticle

Abstract

-To determine whether the formation and maintenance of focal adhesions and costameres in cardiac myocytes are influenced by the mechanical forces that they transmit, we mechanically unloaded these cells by inhibiting their spontaneous contractile activity with the calcium-channel blocker nifedipine (12 μM). Interferencereflection and fluorescence microscopy revealed that within 24 h of arrest, β1-integrin- and vinculin-positive focal adhesions and costameres were disrupted. Loss of mature β1-integrin from the cell surface was observed in cell surfacelabeling experiments and in Western blots. Subjecting nonbeating cells to a 5% static stretch for 24 h resulted in an increase of 21% for β1-integrin and 39% for vinculin. Stretching beating cells resulted in 71 and 9% increases, respectively. Intracellular concentrations of pre-β1 were not affected by contractile activity or by stretch. Our results indicate that mechanical forces stabilize the cellular levels of β1-integrin and vinculin by possibly regulating their association with the formation and maintenance of focal adhesions and costameres. β1-integrin; vinculin

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume273
Issue number2
StatePublished - 1997

Fingerprint

Costameres
Focal Adhesions
Vinculin
Cardiac Myocytes
Integrins
Maintenance
Calcium Channel Blockers
Nifedipine
Fluorescence Microscopy
Western Blotting

Keywords

  • β-integrin
  • Vinculin

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes. / Sharp, Will W.; Simpson, David G.; Borg, Thomas K.; Samarel, Allen M.; Terracio, Louis.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 273, No. 2, 1997.

Research output: Contribution to journalArticle

@article{35b1703a198541c484cc96b8ff781a99,
title = "Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes",
abstract = "-To determine whether the formation and maintenance of focal adhesions and costameres in cardiac myocytes are influenced by the mechanical forces that they transmit, we mechanically unloaded these cells by inhibiting their spontaneous contractile activity with the calcium-channel blocker nifedipine (12 μM). Interferencereflection and fluorescence microscopy revealed that within 24 h of arrest, β1-integrin- and vinculin-positive focal adhesions and costameres were disrupted. Loss of mature β1-integrin from the cell surface was observed in cell surfacelabeling experiments and in Western blots. Subjecting nonbeating cells to a 5{\%} static stretch for 24 h resulted in an increase of 21{\%} for β1-integrin and 39{\%} for vinculin. Stretching beating cells resulted in 71 and 9{\%} increases, respectively. Intracellular concentrations of pre-β1 were not affected by contractile activity or by stretch. Our results indicate that mechanical forces stabilize the cellular levels of β1-integrin and vinculin by possibly regulating their association with the formation and maintenance of focal adhesions and costameres. β1-integrin; vinculin",
keywords = "β-integrin, Vinculin",
author = "Sharp, {Will W.} and Simpson, {David G.} and Borg, {Thomas K.} and Samarel, {Allen M.} and Louis Terracio",
year = "1997",
language = "English (US)",
volume = "273",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Mechanical forces regulate focal adhesion and costamere assembly in cardiac myocytes

AU - Sharp, Will W.

AU - Simpson, David G.

AU - Borg, Thomas K.

AU - Samarel, Allen M.

AU - Terracio, Louis

PY - 1997

Y1 - 1997

N2 - -To determine whether the formation and maintenance of focal adhesions and costameres in cardiac myocytes are influenced by the mechanical forces that they transmit, we mechanically unloaded these cells by inhibiting their spontaneous contractile activity with the calcium-channel blocker nifedipine (12 μM). Interferencereflection and fluorescence microscopy revealed that within 24 h of arrest, β1-integrin- and vinculin-positive focal adhesions and costameres were disrupted. Loss of mature β1-integrin from the cell surface was observed in cell surfacelabeling experiments and in Western blots. Subjecting nonbeating cells to a 5% static stretch for 24 h resulted in an increase of 21% for β1-integrin and 39% for vinculin. Stretching beating cells resulted in 71 and 9% increases, respectively. Intracellular concentrations of pre-β1 were not affected by contractile activity or by stretch. Our results indicate that mechanical forces stabilize the cellular levels of β1-integrin and vinculin by possibly regulating their association with the formation and maintenance of focal adhesions and costameres. β1-integrin; vinculin

AB - -To determine whether the formation and maintenance of focal adhesions and costameres in cardiac myocytes are influenced by the mechanical forces that they transmit, we mechanically unloaded these cells by inhibiting their spontaneous contractile activity with the calcium-channel blocker nifedipine (12 μM). Interferencereflection and fluorescence microscopy revealed that within 24 h of arrest, β1-integrin- and vinculin-positive focal adhesions and costameres were disrupted. Loss of mature β1-integrin from the cell surface was observed in cell surfacelabeling experiments and in Western blots. Subjecting nonbeating cells to a 5% static stretch for 24 h resulted in an increase of 21% for β1-integrin and 39% for vinculin. Stretching beating cells resulted in 71 and 9% increases, respectively. Intracellular concentrations of pre-β1 were not affected by contractile activity or by stretch. Our results indicate that mechanical forces stabilize the cellular levels of β1-integrin and vinculin by possibly regulating their association with the formation and maintenance of focal adhesions and costameres. β1-integrin; vinculin

KW - β-integrin

KW - Vinculin

UR - http://www.scopus.com/inward/record.url?scp=33751297308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751297308&partnerID=8YFLogxK

M3 - Article

C2 - 9277468

AN - SCOPUS:0030752634

VL - 273

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 2

ER -