mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans.

C. Savage, M. Hamelin, J. G. Culotti, A. Coulson, Donna Albertson, M. Chalfie

Research output: Contribution to journalArticle

Abstract

In the nematode Caenorhabditis elegans, microtubules with 15 protofilaments are a specialized feature of six touch-receptor neurons; microtubules found in other C. elegans neurons have 11 protofilaments. Mutations in the gene mec-7 result in touch-insensitive animals whose touch cells lack the 15-protofilament microtubules. We have characterized 54 mutations in the mec-7 gene. The absence of mec-7 activity results selectively in the recessive loss of touch sensitivity. Partial loss-of-function alleles result in a partial loss of touch sensitivity. Dominant mutations, which are isolated at an unusually high proportion, may encode abnormal products. We have cloned the mec-7 gene; it encodes a beta-tubulin which is 90-93% identical to vertebrate beta-tubulin. Our results are consistent with the hypothesis that tubulin heterogeneity contributes to the formation of structurally and functionally distinct sets of microtubules.

Original languageEnglish (US)
Pages (from-to)870-881
Number of pages12
JournalGenes & development
Volume3
Issue number6
StatePublished - Jun 1989

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Caenorhabditis elegans
Touch
Tubulin
Microtubules
Genes
Mutation
Neurons
Vertebrates
Alleles

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans. / Savage, C.; Hamelin, M.; Culotti, J. G.; Coulson, A.; Albertson, Donna; Chalfie, M.

In: Genes & development, Vol. 3, No. 6, 06.1989, p. 870-881.

Research output: Contribution to journalArticle

Savage, C. ; Hamelin, M. ; Culotti, J. G. ; Coulson, A. ; Albertson, Donna ; Chalfie, M. / mec-7 is a beta-tubulin gene required for the production of 15-protofilament microtubules in Caenorhabditis elegans. In: Genes & development. 1989 ; Vol. 3, No. 6. pp. 870-881.
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