Measurement of the elasticity of the actin tail of Listeria monocytogenes

Fabien Gerbal, Valérie Laurent, Albrecht Ott, Marie France Carlier, Paul Chaikin, Jacques Prost

    Research output: Contribution to journalArticle

    Abstract

    We report biophysical experiments performed on the bacterium Listeria monocytogenes, a model system to study actin-based motility. Using optical tweezers and electrophoresis experiments, we find that the bacterium is firmly attached to its tail, and we demonstrate that the tail responds as an elastic gel when deformed. We have measured its elastic modulus at a value of 103-104 Pa, which is 10 times higher than the rigidity of the eukaryotic cytoplasm. These results demonstrate that the bacterium and its tail form a very robust system, consistent with the steadyness of the motion observed in vivo. We propose an elastic model for the propulsion mechanism which takes into account the connection and thus the interaction between the actin filaments. It provides a generic description of the various aspects of actin-tail based movements.

    Original languageEnglish (US)
    Pages (from-to)134-140
    Number of pages7
    JournalEuropean Biophysics Journal
    Volume29
    Issue number2
    StatePublished - 2000

    Fingerprint

    Elasticity
    Listeria monocytogenes
    Actins
    Bacteria
    Optical Tweezers
    Elastic Modulus
    Actin Cytoskeleton
    Electrophoresis
    Cytoplasm
    Gels

    Keywords

    • Actin gel
    • Cell mechanics
    • Cell motility
    • Elasticity
    • Listeria monocytogenes
    • Optical tweezers

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Gerbal, F., Laurent, V., Ott, A., Carlier, M. F., Chaikin, P., & Prost, J. (2000). Measurement of the elasticity of the actin tail of Listeria monocytogenes. European Biophysics Journal, 29(2), 134-140.

    Measurement of the elasticity of the actin tail of Listeria monocytogenes. / Gerbal, Fabien; Laurent, Valérie; Ott, Albrecht; Carlier, Marie France; Chaikin, Paul; Prost, Jacques.

    In: European Biophysics Journal, Vol. 29, No. 2, 2000, p. 134-140.

    Research output: Contribution to journalArticle

    Gerbal, F, Laurent, V, Ott, A, Carlier, MF, Chaikin, P & Prost, J 2000, 'Measurement of the elasticity of the actin tail of Listeria monocytogenes', European Biophysics Journal, vol. 29, no. 2, pp. 134-140.
    Gerbal F, Laurent V, Ott A, Carlier MF, Chaikin P, Prost J. Measurement of the elasticity of the actin tail of Listeria monocytogenes. European Biophysics Journal. 2000;29(2):134-140.
    Gerbal, Fabien ; Laurent, Valérie ; Ott, Albrecht ; Carlier, Marie France ; Chaikin, Paul ; Prost, Jacques. / Measurement of the elasticity of the actin tail of Listeria monocytogenes. In: European Biophysics Journal. 2000 ; Vol. 29, No. 2. pp. 134-140.
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    AU - Prost, Jacques

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