Maturation of intrinsic and synaptic properties of layer 2/3 pyramidal neurons in mouse auditory cortex

Anne Marie M Oswald, Alexander Reyes

Research output: Contribution to journalArticle

Abstract

We investigated the development of L2/3 pyramidal cell (PC) circuitry in juvenile mice from postnatal day 10 (P10) to P29. Using whole cell recordings in an in vitro thalamocortical slice preparation, we examined the connection architecture and intrinsic and synaptic properties of PCs. The excitatory connections between PCs were highly localized: the probability of connection between PCs declined with intersomatic distance from 0.18 to about 0.05 over 150 μm, but did not vary with age. However, the mean and variance of the intrinsic and synaptic properties of PCs changed dramatically between P10 and P29. The input resistance, membrane time constant, and resting membrane potential decreased, leading to reduced neural excitability in older animals. Likewise, there were age-dependent decreases in the amplitude and decay time of the excitatory postsynaptic potentials as well as short-term synaptic depression. Both the intrinsic and synaptic properties underwent a transitional period between P10 and P18 prior to reaching steady state at P19-P29. We show that these properties combine to produce age-related differential synaptic responses to low- and high-frequency synaptic input that may contribute to differences in auditory processing during development.

Original languageEnglish (US)
Pages (from-to)2998-3008
Number of pages11
JournalJournal of Neurophysiology
Volume99
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

Auditory Cortex
Pyramidal Cells
Excitatory Postsynaptic Potentials
Patch-Clamp Techniques
Membrane Potentials
Membranes
In Vitro Techniques

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Maturation of intrinsic and synaptic properties of layer 2/3 pyramidal neurons in mouse auditory cortex. / Oswald, Anne Marie M; Reyes, Alexander.

In: Journal of Neurophysiology, Vol. 99, No. 6, 06.2008, p. 2998-3008.

Research output: Contribution to journalArticle

@article{33663b0ba29849159d6c28810e53488e,
title = "Maturation of intrinsic and synaptic properties of layer 2/3 pyramidal neurons in mouse auditory cortex",
abstract = "We investigated the development of L2/3 pyramidal cell (PC) circuitry in juvenile mice from postnatal day 10 (P10) to P29. Using whole cell recordings in an in vitro thalamocortical slice preparation, we examined the connection architecture and intrinsic and synaptic properties of PCs. The excitatory connections between PCs were highly localized: the probability of connection between PCs declined with intersomatic distance from 0.18 to about 0.05 over 150 μm, but did not vary with age. However, the mean and variance of the intrinsic and synaptic properties of PCs changed dramatically between P10 and P29. The input resistance, membrane time constant, and resting membrane potential decreased, leading to reduced neural excitability in older animals. Likewise, there were age-dependent decreases in the amplitude and decay time of the excitatory postsynaptic potentials as well as short-term synaptic depression. Both the intrinsic and synaptic properties underwent a transitional period between P10 and P18 prior to reaching steady state at P19-P29. We show that these properties combine to produce age-related differential synaptic responses to low- and high-frequency synaptic input that may contribute to differences in auditory processing during development.",
author = "Oswald, {Anne Marie M} and Alexander Reyes",
year = "2008",
month = "6",
doi = "10.1152/jn.01160.2007",
language = "English (US)",
volume = "99",
pages = "2998--3008",
journal = "Journal of Neurophysiology",
issn = "0022-3077",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - Maturation of intrinsic and synaptic properties of layer 2/3 pyramidal neurons in mouse auditory cortex

AU - Oswald, Anne Marie M

AU - Reyes, Alexander

PY - 2008/6

Y1 - 2008/6

N2 - We investigated the development of L2/3 pyramidal cell (PC) circuitry in juvenile mice from postnatal day 10 (P10) to P29. Using whole cell recordings in an in vitro thalamocortical slice preparation, we examined the connection architecture and intrinsic and synaptic properties of PCs. The excitatory connections between PCs were highly localized: the probability of connection between PCs declined with intersomatic distance from 0.18 to about 0.05 over 150 μm, but did not vary with age. However, the mean and variance of the intrinsic and synaptic properties of PCs changed dramatically between P10 and P29. The input resistance, membrane time constant, and resting membrane potential decreased, leading to reduced neural excitability in older animals. Likewise, there were age-dependent decreases in the amplitude and decay time of the excitatory postsynaptic potentials as well as short-term synaptic depression. Both the intrinsic and synaptic properties underwent a transitional period between P10 and P18 prior to reaching steady state at P19-P29. We show that these properties combine to produce age-related differential synaptic responses to low- and high-frequency synaptic input that may contribute to differences in auditory processing during development.

AB - We investigated the development of L2/3 pyramidal cell (PC) circuitry in juvenile mice from postnatal day 10 (P10) to P29. Using whole cell recordings in an in vitro thalamocortical slice preparation, we examined the connection architecture and intrinsic and synaptic properties of PCs. The excitatory connections between PCs were highly localized: the probability of connection between PCs declined with intersomatic distance from 0.18 to about 0.05 over 150 μm, but did not vary with age. However, the mean and variance of the intrinsic and synaptic properties of PCs changed dramatically between P10 and P29. The input resistance, membrane time constant, and resting membrane potential decreased, leading to reduced neural excitability in older animals. Likewise, there were age-dependent decreases in the amplitude and decay time of the excitatory postsynaptic potentials as well as short-term synaptic depression. Both the intrinsic and synaptic properties underwent a transitional period between P10 and P18 prior to reaching steady state at P19-P29. We show that these properties combine to produce age-related differential synaptic responses to low- and high-frequency synaptic input that may contribute to differences in auditory processing during development.

UR - http://www.scopus.com/inward/record.url?scp=57049181979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049181979&partnerID=8YFLogxK

U2 - 10.1152/jn.01160.2007

DO - 10.1152/jn.01160.2007

M3 - Article

VL - 99

SP - 2998

EP - 3008

JO - Journal of Neurophysiology

JF - Journal of Neurophysiology

SN - 0022-3077

IS - 6

ER -