Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis

Cristina Teixeira, Ramesh Rajpurohit, Kyle Mansfield, Yelena V. Nemelivsky, Irving M. Shapiro

Research output: Contribution to journalArticle

Abstract

The major aim of the current investigation was to evaluate the role of thiols during chondrocyte maturation and apoptosis. Using a thiol-sensitive fluorescent probe, we found that in chick growth plate chondrocytes, hypertrophy is accompanied by a decrease in the glutathione content. In this study, we show that the maturation-dependent loss of thiol, although not causing death of maturing chondrocytes, drastically increases susceptibility to apoptosis by oxidative and nitrosoactive stress. To investigate how the loss of thiol content in cultured chondrocytes affects the expression of the hypertrophic phenotype, we chemically manipulated intracellular thiol levels and analyzed the expression of important maturation markers. We found that thiol depletion causes a decrease in the expression of osteopontin, type X and type II collagen and a significant loss of alkaline phosphatase activity, suggesting that the expression of the hypertrophic phenotype is tightly regulated by redox levels in chondrocytes. Furthermore, severe thiol depletion profoundly affected cell survival under oxidative and nitrosoactive stress. It was concluded that the loss of thiol reserve is not only linked to the expression of the hypertrophic phenotype but also influenced chondrocyte survival, linking chondrocyte maturation and the activation of the apoptotic pathway.

Original languageEnglish (US)
Pages (from-to)662-668
Number of pages7
JournalJournal of Bone and Mineral Research
Volume18
Issue number4
StatePublished - Apr 1 2003

Fingerprint

Chondrocytes
Sulfhydryl Compounds
Apoptosis
Phenotype
Oxidative Stress
Collagen Type X
Osteopontin
Growth Plate
Collagen Type II
Fluorescent Dyes
Hypertrophy
Oxidation-Reduction
Glutathione
Alkaline Phosphatase
Cell Survival

Keywords

  • Apoptosis
  • Chondrocyte maturation
  • Gene expression
  • Glutathione
  • Retinoic acid

ASJC Scopus subject areas

  • Surgery

Cite this

Teixeira, C., Rajpurohit, R., Mansfield, K., Nemelivsky, Y. V., & Shapiro, I. M. (2003). Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis. Journal of Bone and Mineral Research, 18(4), 662-668.

Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis. / Teixeira, Cristina; Rajpurohit, Ramesh; Mansfield, Kyle; Nemelivsky, Yelena V.; Shapiro, Irving M.

In: Journal of Bone and Mineral Research, Vol. 18, No. 4, 01.04.2003, p. 662-668.

Research output: Contribution to journalArticle

Teixeira, C, Rajpurohit, R, Mansfield, K, Nemelivsky, YV & Shapiro, IM 2003, 'Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis', Journal of Bone and Mineral Research, vol. 18, no. 4, pp. 662-668.
Teixeira C, Rajpurohit R, Mansfield K, Nemelivsky YV, Shapiro IM. Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis. Journal of Bone and Mineral Research. 2003 Apr 1;18(4):662-668.
Teixeira, Cristina ; Rajpurohit, Ramesh ; Mansfield, Kyle ; Nemelivsky, Yelena V. ; Shapiro, Irving M. / Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis. In: Journal of Bone and Mineral Research. 2003 ; Vol. 18, No. 4. pp. 662-668.
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