Mass-based readout for agglutination assays

Rumi Chunara, Michel Godin, Scott M. Knudsen, Scott R. Manalis

Research output: Contribution to journalArticle

Abstract

We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

Original languageEnglish (US)
Article number193902
JournalApplied Physics Letters
Volume91
Issue number19
DOIs
StatePublished - 2007

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agglutination
readout
cytometry
microchannels
antibodies
image analysis
monomers
resonators
dimers
dosage
curves

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Chunara, R., Godin, M., Knudsen, S. M., & Manalis, S. R. (2007). Mass-based readout for agglutination assays. Applied Physics Letters, 91(19), [193902]. https://doi.org/10.1063/1.2806197

Mass-based readout for agglutination assays. / Chunara, Rumi; Godin, Michel; Knudsen, Scott M.; Manalis, Scott R.

In: Applied Physics Letters, Vol. 91, No. 19, 193902, 2007.

Research output: Contribution to journalArticle

Chunara, R, Godin, M, Knudsen, SM & Manalis, SR 2007, 'Mass-based readout for agglutination assays', Applied Physics Letters, vol. 91, no. 19, 193902. https://doi.org/10.1063/1.2806197
Chunara, Rumi ; Godin, Michel ; Knudsen, Scott M. ; Manalis, Scott R. / Mass-based readout for agglutination assays. In: Applied Physics Letters. 2007 ; Vol. 91, No. 19.
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