Mass-based readout for agglutination assays

Rumi Chunara; Michel Godin; Scott M. Knudsen; Scott R. Manalis

doi:10.1063/1.2806197

Mass-based readout for agglutination assays

Rumi Chunara, Michel Godin, Scott M. Knudsen, Scott R. Manalis

Global Public Health

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Abstract

We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.

Original language	English (US)
Article number	193902
Journal	Applied Physics Letters
Volume	91
Issue number	19
DOIs	https://doi.org/10.1063/1.2806197
State	Published - Nov 19 2007

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ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

Cite this

Chunara, R., Godin, M., Knudsen, S. M., & Manalis, S. R. (2007). Mass-based readout for agglutination assays. Applied Physics Letters, 91(19), [193902]. https://doi.org/10.1063/1.2806197

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10.1063/1.2806197