Abstract
We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.
| Original language | English (US) |
|---|---|
| Article number | 193902 |
| Journal | Applied Physics Letters |
| Volume | 91 |
| Issue number | 19 |
| DOIs | |
| State | Published - 2007 |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Mass-based readout for agglutination assays. / Chunara, Rumi; Godin, Michel; Knudsen, Scott M.; Manalis, Scott R.
In: Applied Physics Letters, Vol. 91, No. 19, 193902, 2007.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mass-based readout for agglutination assays
AU - Chunara, Rumi
AU - Godin, Michel
AU - Knudsen, Scott M.
AU - Manalis, Scott R.
PY - 2007
Y1 - 2007
N2 - We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.
AB - We present a mass-based readout for agglutination assays. The suspended microchannel resonator (SMR) is used to classify monomers and dimers that are formed during early stage aggregation, and to relate the total count to the analyte concentration. Using a model system of streptavidin functionalized microspheres and biotinylated antibody as the analyte, we obtain a dose-response curve over a concentration range of 0.63-630 nM and show that the results are comparable to what has been previously achieved by image analysis and conventional flow cytometry.
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UR - http://www.scopus.com/inward/citedby.url?scp=36049043553&partnerID=8YFLogxK
U2 - 10.1063/1.2806197
DO - 10.1063/1.2806197
M3 - Article
VL - 91
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 19
M1 - 193902
ER -