Mapping segmental and sequence variations among laboratory mice using BAC array CGH

Antoine M. Snijders, Norma J. Nowak, Bing Huey, Jane Fridlyand, Sindy Law, Jeffrey Conroy, Taku Tokuyasu, Kubilay Demir, Readman Chiu, Jian Hua Mao, Ajay N. Jain, Steven J M Jones, Allan Balmain, Daniel Pinkel, Donna Albertson

Research output: Contribution to journalArticle

Abstract

We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation ( log2ratio ≥ 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Δ log2ratio ∼0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.

Original languageEnglish (US)
Pages (from-to)302-311
Number of pages10
JournalGenome Research
Volume15
Issue number2
DOIs
StatePublished - 2005

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Clone Cells
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 1
Genome

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  • Genetics

Cite this

Snijders, A. M., Nowak, N. J., Huey, B., Fridlyand, J., Law, S., Conroy, J., ... Albertson, D. (2005). Mapping segmental and sequence variations among laboratory mice using BAC array CGH. Genome Research, 15(2), 302-311. https://doi.org/10.1101/gr.2902505

Mapping segmental and sequence variations among laboratory mice using BAC array CGH. / Snijders, Antoine M.; Nowak, Norma J.; Huey, Bing; Fridlyand, Jane; Law, Sindy; Conroy, Jeffrey; Tokuyasu, Taku; Demir, Kubilay; Chiu, Readman; Mao, Jian Hua; Jain, Ajay N.; Jones, Steven J M; Balmain, Allan; Pinkel, Daniel; Albertson, Donna.

In: Genome Research, Vol. 15, No. 2, 2005, p. 302-311.

Research output: Contribution to journalArticle

Snijders, AM, Nowak, NJ, Huey, B, Fridlyand, J, Law, S, Conroy, J, Tokuyasu, T, Demir, K, Chiu, R, Mao, JH, Jain, AN, Jones, SJM, Balmain, A, Pinkel, D & Albertson, D 2005, 'Mapping segmental and sequence variations among laboratory mice using BAC array CGH', Genome Research, vol. 15, no. 2, pp. 302-311. https://doi.org/10.1101/gr.2902505
Snijders AM, Nowak NJ, Huey B, Fridlyand J, Law S, Conroy J et al. Mapping segmental and sequence variations among laboratory mice using BAC array CGH. Genome Research. 2005;15(2):302-311. https://doi.org/10.1101/gr.2902505
Snijders, Antoine M. ; Nowak, Norma J. ; Huey, Bing ; Fridlyand, Jane ; Law, Sindy ; Conroy, Jeffrey ; Tokuyasu, Taku ; Demir, Kubilay ; Chiu, Readman ; Mao, Jian Hua ; Jain, Ajay N. ; Jones, Steven J M ; Balmain, Allan ; Pinkel, Daniel ; Albertson, Donna. / Mapping segmental and sequence variations among laboratory mice using BAC array CGH. In: Genome Research. 2005 ; Vol. 15, No. 2. pp. 302-311.
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