Many ribosomal protein genes are cancer genes in zebrafish

Adam Amsterdam, Kirsten Sadler Edepli, Kevin Lai, Sarah Farrington, Roderick T. Bronson, Jacqueline A. Lees, Nancy Hopkins

Research output: Contribution to journalArticle

Abstract

We have generated several hundred lines of zebrafish (Danio rerio), each heterozygous for a recessive embryonic lethal mutation. Since many tumor suppressor genes are recessive lethals, we screened our colony for lines that display early mortality and/or gross evidence of tumors. We identified 12 lines with elevated cancer incidence. Fish from these lines develop malignant peripheral nerve sheath tumors, and in some cases also other tumor types, with moderate to very high frequencies. Surprisingly, 11 of the 12 lines were each heterozygous for a mutation in a different ribosomal protein (RP) gene, while one line was heterozygous for a mutation in a zebrafish paralog of the human and mouse tumor suppressor gene, neurofibromatosis type 2. Our findings suggest that many RP genes may act as haploinsufficient tumor suppressors in fish. Many RP genes might also be cancer genes in humans, where their role in tumorigenesis could easily have escaped detection up to now.

Original languageEnglish (US)
JournalPLoS Biology
Volume2
Issue number5
DOIs
StatePublished - Sep 27 2004

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Ribosomal Proteins
Neoplasm Genes
ribosomal proteins
Zebrafish
Danio rerio
Tumors
Genes
neoplasms
Tumor Suppressor Genes
Mutation
Neoplasms
Fishes
tumor suppressor genes
genes
Neurofibromatosis 2
mutation
lethal genes
Fish
Neurilemmoma
Carcinogenesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Amsterdam, A., Sadler Edepli, K., Lai, K., Farrington, S., Bronson, R. T., Lees, J. A., & Hopkins, N. (2004). Many ribosomal protein genes are cancer genes in zebrafish. PLoS Biology, 2(5). https://doi.org/10.1371/journal.pbio.0020139

Many ribosomal protein genes are cancer genes in zebrafish. / Amsterdam, Adam; Sadler Edepli, Kirsten; Lai, Kevin; Farrington, Sarah; Bronson, Roderick T.; Lees, Jacqueline A.; Hopkins, Nancy.

In: PLoS Biology, Vol. 2, No. 5, 27.09.2004.

Research output: Contribution to journalArticle

Amsterdam, A, Sadler Edepli, K, Lai, K, Farrington, S, Bronson, RT, Lees, JA & Hopkins, N 2004, 'Many ribosomal protein genes are cancer genes in zebrafish', PLoS Biology, vol. 2, no. 5. https://doi.org/10.1371/journal.pbio.0020139
Amsterdam, Adam ; Sadler Edepli, Kirsten ; Lai, Kevin ; Farrington, Sarah ; Bronson, Roderick T. ; Lees, Jacqueline A. ; Hopkins, Nancy. / Many ribosomal protein genes are cancer genes in zebrafish. In: PLoS Biology. 2004 ; Vol. 2, No. 5.
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