Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors

Romain Durand De Cuttoli, Sarah Mondoloni, Fabio Marti, Damien Lemoine, Claire Nguyen, Jérémie Naudé, Thibaut D’izarny-Gargas, Stéphanie Pons, Uwe Maskos, Dirk Trauner, Richard H. Kramer, Philippe Faure, Alexandre Mourot

Research output: Contribution to journalArticle

Abstract

Dopamine (DA) neurons of the ventral tegmental area (VTA) integrate cholinergic inputs to regulate key functions such as motivation and goal-directed behaviors. Yet the temporal dynamic range and mechanism of action of acetylcholine (ACh) on the modulation of VTA circuits and reward-related behaviors are not known. Here, we used a chemical-genetic approach for rapid and precise optical manipulation of nicotinic neurotransmission in VTA neurons in living mice. We provide direct evidence that the ACh tone fine-tunes the firing properties of VTA DA neurons through β2-containing (β2*) nicotinic ACh receptors (nAChRs). Furthermore, locally photo-antagonizing these receptors in the VTA was sufficient to reversibly switch nicotine reinforcement on and off. By enabling control of nicotinic transmission in targeted brain circuits, this technology will help unravel the various physiological functions of nAChRs and may assist in the design of novel therapies relevant to neuropsychiatric disorders.

Original languageEnglish (US)
Article numbere37487
JournaleLife
Volume7
DOIs
StatePublished - Sep 1 2018

Fingerprint

Ventral Tegmental Area
Dopaminergic Neurons
Nicotinic Receptors
Mesencephalon
Reward
Neurons
Dopamine
Light
Acetylcholine
Networks (circuits)
Cholinergic Receptors
Nicotine
Cholinergic Agents
Brain
Reinforcement
Switches
Modulation
Synaptic Transmission
Motivation
Technology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Cuttoli, R. D. D., Mondoloni, S., Marti, F., Lemoine, D., Nguyen, C., Naudé, J., ... Mourot, A. (2018). Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. eLife, 7, [e37487]. https://doi.org/10.7554/eLife.37487

Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. / Cuttoli, Romain Durand De; Mondoloni, Sarah; Marti, Fabio; Lemoine, Damien; Nguyen, Claire; Naudé, Jérémie; D’izarny-Gargas, Thibaut; Pons, Stéphanie; Maskos, Uwe; Trauner, Dirk; Kramer, Richard H.; Faure, Philippe; Mourot, Alexandre.

In: eLife, Vol. 7, e37487, 01.09.2018.

Research output: Contribution to journalArticle

Cuttoli, RDD, Mondoloni, S, Marti, F, Lemoine, D, Nguyen, C, Naudé, J, D’izarny-Gargas, T, Pons, S, Maskos, U, Trauner, D, Kramer, RH, Faure, P & Mourot, A 2018, 'Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors', eLife, vol. 7, e37487. https://doi.org/10.7554/eLife.37487
Cuttoli, Romain Durand De ; Mondoloni, Sarah ; Marti, Fabio ; Lemoine, Damien ; Nguyen, Claire ; Naudé, Jérémie ; D’izarny-Gargas, Thibaut ; Pons, Stéphanie ; Maskos, Uwe ; Trauner, Dirk ; Kramer, Richard H. ; Faure, Philippe ; Mourot, Alexandre. / Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors. In: eLife. 2018 ; Vol. 7.
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