Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model

N. Alikhani, R. D. Ferguson, R. Novosyadlyy, E. J. Gallagher, E. J. Scheinman, Shoshana Yakar, D. Leroith

Research output: Contribution to journalArticle

Abstract

Dyslipidemia has been associated with an increased risk for developing cancer. However, the implicated mechanisms are largely unknown. To explore the role of dyslipidemia in breast cancer growth and metastasis, we used the apolipoprotein E (ApoE) knockout mice (ApoE -/-), which exhibit marked dyslipidemia, with elevated circulating cholesterol and triglyceride levels in the setting of normal glucose homeostasis and insulin sensitivity. Non-metastatic Met-1 and metastatic Mvt-1 mammary cancer cells derived from MMTV-PyVmT/FVB-N transgenic mice and c-Myc/vegf tumor explants respectively, were injected into the mammary fat pad of ApoE -/- and wild-type (WT) females consuming a high-fat/high-cholesterol diet and tumor growth was evaluated. ApoE -/- mice exhibited increased tumor growth and displayed a greater number of spontaneous metastases to the lungs. Furthermore, intravenous injection of Mvt-1 cells resulted in a greater number of pulmonary metastases in the lungs of ApoE -/- mice compared with WT controls. To unravel the molecular mechanism involved in enhanced tumor growth in ApoE -/- mice, we studied the response of Mvt-1 cells to cholesterol in vitro. We found that cholesterol increased AktS473 phosphorylation in Mvt-1 cells as well as cellular proliferation, whereas cholesterol depletion in the cell membrane abrogated AktS473 phosphorylation induced by exogenously added cholesterol. Furthermore, in vivo administration of BKM120, a small-molecule inhibitor of phosphatidylinositol 3-kinase (PI3K), alleviated dyslipidemia-induced tumor growth and metastasis in Mvt-1 model with a concomitant decrease in PI3K/Akt signaling. Collectively, we suggest that the hypercholesterolemic milieu in the ApoE -/- mice is a favorable setting for mammary tumor growth and metastasis.

Original languageEnglish (US)
Pages (from-to)961-967
Number of pages7
JournalOncogene
Volume32
Issue number8
DOIs
StatePublished - Feb 21 2013

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Apolipoproteins E
Breast Neoplasms
Neoplasm Metastasis
Lung
Dyslipidemias
Cholesterol
Growth
Phosphatidylinositol 3-Kinase
Neoplasms
Phosphorylation
Cell Proliferation
High Fat Diet
Hypercholesterolemia
Knockout Mice
Intravenous Injections
Vascular Endothelial Growth Factor A
Transgenic Mice
Insulin Resistance
Adipose Tissue
Triglycerides

Keywords

  • Akt
  • ApoE
  • dyslipidemia
  • mammary tumor
  • metastasis

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Alikhani, N., Ferguson, R. D., Novosyadlyy, R., Gallagher, E. J., Scheinman, E. J., Yakar, S., & Leroith, D. (2013). Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model. Oncogene, 32(8), 961-967. https://doi.org/10.1038/onc.2012.113

Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model. / Alikhani, N.; Ferguson, R. D.; Novosyadlyy, R.; Gallagher, E. J.; Scheinman, E. J.; Yakar, Shoshana; Leroith, D.

In: Oncogene, Vol. 32, No. 8, 21.02.2013, p. 961-967.

Research output: Contribution to journalArticle

Alikhani, N, Ferguson, RD, Novosyadlyy, R, Gallagher, EJ, Scheinman, EJ, Yakar, S & Leroith, D 2013, 'Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model', Oncogene, vol. 32, no. 8, pp. 961-967. https://doi.org/10.1038/onc.2012.113
Alikhani N, Ferguson RD, Novosyadlyy R, Gallagher EJ, Scheinman EJ, Yakar S et al. Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model. Oncogene. 2013 Feb 21;32(8):961-967. https://doi.org/10.1038/onc.2012.113
Alikhani, N. ; Ferguson, R. D. ; Novosyadlyy, R. ; Gallagher, E. J. ; Scheinman, E. J. ; Yakar, Shoshana ; Leroith, D. / Mammary tumor growth and pulmonary metastasis are enhanced in a hyperlipidemic mouse model. In: Oncogene. 2013 ; Vol. 32, No. 8. pp. 961-967.
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