Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus

Ji Young Min, Celia Santos, Adam Fitch, Alan Twaddle, Yoshiko Toyoda, Jay V. DePasse, Elodie Ghedin, Kanta Subbarao

Research output: Contribution to journalArticle

Abstract

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Original languageEnglish (US)
Pages (from-to)10884-10888
Number of pages5
JournalJournal of Virology
Volume87
Issue number19
DOIs
StatePublished - 2013

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H5N1 Subtype Influenza A Virus
Influenza in Birds
Orthomyxoviridae
Avian Proteins
mutation
Mutation
Host Specificity
mice
Virus Replication
virus replication
glutamic acid
Influenza A virus
respiratory system
host range
Respiratory System
Genes
Lysine
Virulence
Glutamic Acid
Mammals

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Min, J. Y., Santos, C., Fitch, A., Twaddle, A., Toyoda, Y., DePasse, J. V., ... Subbarao, K. (2013). Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus. Journal of Virology, 87(19), 10884-10888. https://doi.org/10.1128/JVI.01016-13

Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus. / Min, Ji Young; Santos, Celia; Fitch, Adam; Twaddle, Alan; Toyoda, Yoshiko; DePasse, Jay V.; Ghedin, Elodie; Subbarao, Kanta.

In: Journal of Virology, Vol. 87, No. 19, 2013, p. 10884-10888.

Research output: Contribution to journalArticle

Min, JY, Santos, C, Fitch, A, Twaddle, A, Toyoda, Y, DePasse, JV, Ghedin, E & Subbarao, K 2013, 'Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus', Journal of Virology, vol. 87, no. 19, pp. 10884-10888. https://doi.org/10.1128/JVI.01016-13
Min JY, Santos C, Fitch A, Twaddle A, Toyoda Y, DePasse JV et al. Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus. Journal of Virology. 2013;87(19):10884-10888. https://doi.org/10.1128/JVI.01016-13
Min, Ji Young ; Santos, Celia ; Fitch, Adam ; Twaddle, Alan ; Toyoda, Yoshiko ; DePasse, Jay V. ; Ghedin, Elodie ; Subbarao, Kanta. / Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus. In: Journal of Virology. 2013 ; Vol. 87, No. 19. pp. 10884-10888.
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