Makorin ortholog LEP-2 regulates LIN-28 stability to promote the juvenile-to-adult transition in Caenorhabditis elegans

R. Antonio Herrera, Karin Kiontke, David Fitch

Research output: Contribution to journalArticle

Abstract

The heterochronic genes lin-28, let-7 and lin-41 regulate fundamental developmental transitions in animals, such as stemness versus differentiation and juvenile versus adult states. We identify a new heterochronic gene, lep-2, in Caenorhabditis elegans. Mutations in lep-2 cause a delay in the juvenile-to-adult transition, with adult males retaining pointed, juvenile tail tips, and displaying defective sexual behaviors. In both sexes, lep-2 mutants fail to cease molting or produce an adult cuticle. We find that LEP-2 post-translationally regulates LIN-28 by promoting LIN-28 protein degradation. lep-2 encodes the sole C. elegans ortholog of the Makorin (Mkrn) family of proteins. Like lin-28 and other heterochronic pathway members, vertebrate Mkrns are involved in developmental switches, including the timing of pubertal onset in humans. Based on shared roles, conservation and the interaction between lep-2 and lin-28 shown here, we propose that Mkrns, together with other heterochronic genes, constitute an evolutionarily ancient conserved module regulating switches in development.

Original languageEnglish (US)
Pages (from-to)799-809
Number of pages11
JournalDevelopment
Volume143
Issue number5
DOIs
StatePublished - Mar 1 2016

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Caenorhabditis elegans
Genes
Molting
Sexual Behavior
Proteolysis
Vertebrates
Tail
Mutation
Proteins

Keywords

  • Developmental timing
  • Heterochronic pathway
  • LIN-28/let-7 axis
  • Mkrn

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Makorin ortholog LEP-2 regulates LIN-28 stability to promote the juvenile-to-adult transition in Caenorhabditis elegans. / Antonio Herrera, R.; Kiontke, Karin; Fitch, David.

In: Development, Vol. 143, No. 5, 01.03.2016, p. 799-809.

Research output: Contribution to journalArticle

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