Magnetization transfer in liposome and proteoliposome samples that mimic the protein and lipid composition of myelin

Weiqi Yang, Jae Seung Lee, Maureen Leninger, Johannes Windschuh, Nathaniel Traaseth, Alexej Jerschow

Research output: Contribution to journalArticle

Abstract

Although magnetization transfer (MT) has been widely used in brain MRI, for example in brain inflammation and multiple sclerosis, the detailed molecular origin of MT effects and the role that proteins play in MT remain unclear. In this work, a proteoliposome model system was used to mimic the myelin environment and to examine the roles of protein, cholesterol, brain cerebrosides, and sphingomyelin embedded in the liposome matrix. Exchange parameters were determined using a double-quantum filter experiment. The goal was to determine the relative contributions to exchange and MT of cerebrosides, sphingomyelin, cholesterol, and proteins in 1,2-dimyristoyl-sn-glycero-3-phosphocholine bilayers. The main finding was that cerebrosides produced the strongest exchange effects, and that these were even more pronounced than those found for proteins. Sphingomyelin (which also has exchangeable groups at the head of the fatty acid chains, albeit closer to the lipid acyl chains) and cholesterol showed only minimal transfer. Overall, the extracted exchange rates appeared much smaller than commonly assumed for -OH and -NH groups.

Original languageEnglish (US)
Article numbere4097
JournalNMR in Biomedicine
DOIs
StatePublished - Jan 1 2019

Fingerprint

Cerebrosides
Myelin Sheath
Liposomes
Sphingomyelins
Magnetization
Lipids
Brain
Cholesterol
Chemical analysis
Proteins
Phosphorylcholine
Encephalitis
Magnetic resonance imaging
Multiple Sclerosis
Fatty Acids
proteoliposomes
Experiments

Keywords

  • chemical exchange saturation transfer
  • magnetization transfer
  • white matter diseases

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

Magnetization transfer in liposome and proteoliposome samples that mimic the protein and lipid composition of myelin. / Yang, Weiqi; Lee, Jae Seung; Leninger, Maureen; Windschuh, Johannes; Traaseth, Nathaniel; Jerschow, Alexej.

In: NMR in Biomedicine, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Traaseth, Nathaniel

AU - Jerschow, Alexej

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