Lymphokine Expression Profile of Resting and Stimulated CD4+ CTL Clones Specific for the Glycoprotein of Vesicular Stomatitis Virus

Ben Ning Cao, Brandon S. Huneycutt, Carolina P. Gapud, Robert J. Arceci, Carol Reiss

Research output: Contribution to journalArticle

Abstract

A panel of long-term murine T lymphocyte clones specific for the glycoprotein of vesicular stomatitis virus (VSV) in association with either H-2I-Ad or I-Ed was tested for the production of cytokines in both resting and poststimulation states using both in situ hybridization and bioassay. All but one of the clones showed antigen-specific cytolytic activity in a 4-hr 51Cr release assay. Unexpectedly, the clones did not appear to be typical Th1 cells. Five of these T cell clones produced both IL-2 and IFN-γ but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A). Some clones constitutively expressed mRNA for IL-2 and INF-γ. The proliferation of these clones was factor independent, suggesting an autocrine growth mechanism. Three clones produced variable levels of IL-4 mRNA and some, to significant quantities, of IL-2 mRNA. One cytolytic clone produced neither IL-2 nor IL-4 mRNA to detectable levels, although mRNA for IFN-γ was observed. A noncytolytic, Ag-specific clone produced IL-6, tumor necrosis factor (TNF), and lymphotoxin (LT), but no IL-2, IL-4, or IFN-γ mRNA. There was a strong quantitative correlation between the expression of IL-2-, INF-γ-, and LT-specific mRNAs by the clones. All the T cell clones tested which secreted INF-γ and LT expressed no measurable IL-4 mRNA. We examined expression of several other genes in the panel of clones. These included TNF, met-enkephalin (met-enk), IL-1 and IL-6. IL-1 m-RNA synthesis was not observed in any of the T cell clones. Almost all clones produced TNF mRNA. Parallel bioassays showed that secreted IL-2/IL-4 activity levels and mRNA levels correlated well for all clones. Thus, we observed a great degree of heterogeneity among CD4+ cytolytic T lymphocyte clones.

Original languageEnglish (US)
Pages (from-to)147-156
Number of pages10
JournalCellular Immunology
Volume146
Issue number1
DOIs
StatePublished - Jan 1993

Fingerprint

Vesicular Stomatitis
Lymphokines
Glycoproteins
Clone Cells
Viruses
Interleukin-2
Messenger RNA
Interleukin-4
Lymphotoxin-alpha
T-Lymphocytes
Tumor Necrosis Factor-alpha
Interleukin-1
Biological Assay
Interleukin-6
Methionine Enkephalin
Th1 Cells
Concanavalin A

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Lymphokine Expression Profile of Resting and Stimulated CD4+ CTL Clones Specific for the Glycoprotein of Vesicular Stomatitis Virus. / Cao, Ben Ning; Huneycutt, Brandon S.; Gapud, Carolina P.; Arceci, Robert J.; Reiss, Carol.

In: Cellular Immunology, Vol. 146, No. 1, 01.1993, p. 147-156.

Research output: Contribution to journalArticle

Cao, Ben Ning ; Huneycutt, Brandon S. ; Gapud, Carolina P. ; Arceci, Robert J. ; Reiss, Carol. / Lymphokine Expression Profile of Resting and Stimulated CD4+ CTL Clones Specific for the Glycoprotein of Vesicular Stomatitis Virus. In: Cellular Immunology. 1993 ; Vol. 146, No. 1. pp. 147-156.
@article{8f0c46ea18a04e14a6b78b6757b25499,
title = "Lymphokine Expression Profile of Resting and Stimulated CD4+ CTL Clones Specific for the Glycoprotein of Vesicular Stomatitis Virus",
abstract = "A panel of long-term murine T lymphocyte clones specific for the glycoprotein of vesicular stomatitis virus (VSV) in association with either H-2I-Ad or I-Ed was tested for the production of cytokines in both resting and poststimulation states using both in situ hybridization and bioassay. All but one of the clones showed antigen-specific cytolytic activity in a 4-hr 51Cr release assay. Unexpectedly, the clones did not appear to be typical Th1 cells. Five of these T cell clones produced both IL-2 and IFN-γ but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A). Some clones constitutively expressed mRNA for IL-2 and INF-γ. The proliferation of these clones was factor independent, suggesting an autocrine growth mechanism. Three clones produced variable levels of IL-4 mRNA and some, to significant quantities, of IL-2 mRNA. One cytolytic clone produced neither IL-2 nor IL-4 mRNA to detectable levels, although mRNA for IFN-γ was observed. A noncytolytic, Ag-specific clone produced IL-6, tumor necrosis factor (TNF), and lymphotoxin (LT), but no IL-2, IL-4, or IFN-γ mRNA. There was a strong quantitative correlation between the expression of IL-2-, INF-γ-, and LT-specific mRNAs by the clones. All the T cell clones tested which secreted INF-γ and LT expressed no measurable IL-4 mRNA. We examined expression of several other genes in the panel of clones. These included TNF, met-enkephalin (met-enk), IL-1 and IL-6. IL-1 m-RNA synthesis was not observed in any of the T cell clones. Almost all clones produced TNF mRNA. Parallel bioassays showed that secreted IL-2/IL-4 activity levels and mRNA levels correlated well for all clones. Thus, we observed a great degree of heterogeneity among CD4+ cytolytic T lymphocyte clones.",
author = "Cao, {Ben Ning} and Huneycutt, {Brandon S.} and Gapud, {Carolina P.} and Arceci, {Robert J.} and Carol Reiss",
year = "1993",
month = "1",
doi = "10.1006/cimm.1993.1013",
language = "English (US)",
volume = "146",
pages = "147--156",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Lymphokine Expression Profile of Resting and Stimulated CD4+ CTL Clones Specific for the Glycoprotein of Vesicular Stomatitis Virus

AU - Cao, Ben Ning

AU - Huneycutt, Brandon S.

AU - Gapud, Carolina P.

AU - Arceci, Robert J.

AU - Reiss, Carol

PY - 1993/1

Y1 - 1993/1

N2 - A panel of long-term murine T lymphocyte clones specific for the glycoprotein of vesicular stomatitis virus (VSV) in association with either H-2I-Ad or I-Ed was tested for the production of cytokines in both resting and poststimulation states using both in situ hybridization and bioassay. All but one of the clones showed antigen-specific cytolytic activity in a 4-hr 51Cr release assay. Unexpectedly, the clones did not appear to be typical Th1 cells. Five of these T cell clones produced both IL-2 and IFN-γ but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A). Some clones constitutively expressed mRNA for IL-2 and INF-γ. The proliferation of these clones was factor independent, suggesting an autocrine growth mechanism. Three clones produced variable levels of IL-4 mRNA and some, to significant quantities, of IL-2 mRNA. One cytolytic clone produced neither IL-2 nor IL-4 mRNA to detectable levels, although mRNA for IFN-γ was observed. A noncytolytic, Ag-specific clone produced IL-6, tumor necrosis factor (TNF), and lymphotoxin (LT), but no IL-2, IL-4, or IFN-γ mRNA. There was a strong quantitative correlation between the expression of IL-2-, INF-γ-, and LT-specific mRNAs by the clones. All the T cell clones tested which secreted INF-γ and LT expressed no measurable IL-4 mRNA. We examined expression of several other genes in the panel of clones. These included TNF, met-enkephalin (met-enk), IL-1 and IL-6. IL-1 m-RNA synthesis was not observed in any of the T cell clones. Almost all clones produced TNF mRNA. Parallel bioassays showed that secreted IL-2/IL-4 activity levels and mRNA levels correlated well for all clones. Thus, we observed a great degree of heterogeneity among CD4+ cytolytic T lymphocyte clones.

AB - A panel of long-term murine T lymphocyte clones specific for the glycoprotein of vesicular stomatitis virus (VSV) in association with either H-2I-Ad or I-Ed was tested for the production of cytokines in both resting and poststimulation states using both in situ hybridization and bioassay. All but one of the clones showed antigen-specific cytolytic activity in a 4-hr 51Cr release assay. Unexpectedly, the clones did not appear to be typical Th1 cells. Five of these T cell clones produced both IL-2 and IFN-γ but not IL-4 after stimulation with either phorbol 12-myristate 13-acetate (PMA) or concanavalin A (Con A). Some clones constitutively expressed mRNA for IL-2 and INF-γ. The proliferation of these clones was factor independent, suggesting an autocrine growth mechanism. Three clones produced variable levels of IL-4 mRNA and some, to significant quantities, of IL-2 mRNA. One cytolytic clone produced neither IL-2 nor IL-4 mRNA to detectable levels, although mRNA for IFN-γ was observed. A noncytolytic, Ag-specific clone produced IL-6, tumor necrosis factor (TNF), and lymphotoxin (LT), but no IL-2, IL-4, or IFN-γ mRNA. There was a strong quantitative correlation between the expression of IL-2-, INF-γ-, and LT-specific mRNAs by the clones. All the T cell clones tested which secreted INF-γ and LT expressed no measurable IL-4 mRNA. We examined expression of several other genes in the panel of clones. These included TNF, met-enkephalin (met-enk), IL-1 and IL-6. IL-1 m-RNA synthesis was not observed in any of the T cell clones. Almost all clones produced TNF mRNA. Parallel bioassays showed that secreted IL-2/IL-4 activity levels and mRNA levels correlated well for all clones. Thus, we observed a great degree of heterogeneity among CD4+ cytolytic T lymphocyte clones.

UR - http://www.scopus.com/inward/record.url?scp=0027471694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027471694&partnerID=8YFLogxK

U2 - 10.1006/cimm.1993.1013

DO - 10.1006/cimm.1993.1013

M3 - Article

VL - 146

SP - 147

EP - 156

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -