Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon

Jordan S. Pober, Tucker Collins, Michael A. Gimbrone, Ramzi S. Cotran, Jonathan D. Gitlin, Walter Fiers, Carol Clayberger, Alan M. Krensky, Steven J. Burakoff, Carol Reiss

Research output: Contribution to journalArticle

Abstract

T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1-3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B) 4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5-8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing 'passenger' leukocytes in rodent organ grafts appears necessary for graft rejection9-11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12-15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

Original languageEnglish (US)
Pages (from-to)726-729
Number of pages4
JournalNature
Volume305
Issue number5936
DOIs
StatePublished - 1983

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Histocompatibility Antigens Class II
Interferon-gamma
Blood Vessels
Fibroblasts
Lymphocytes
Human Umbilical Vein Endothelial Cells
Skin
T-Lymphocytes
HLA-A Antigens
HLA-B Antigens
HLA-DQ Antigens
Transplants
Foreskin
Histocompatibility Antigens Class I
Umbilical Veins
Simian virus 40
Isoantigens
Lymphokines
Viral DNA
HLA-DR Antigens

ASJC Scopus subject areas

  • General

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Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. / Pober, Jordan S.; Collins, Tucker; Gimbrone, Michael A.; Cotran, Ramzi S.; Gitlin, Jonathan D.; Fiers, Walter; Clayberger, Carol; Krensky, Alan M.; Burakoff, Steven J.; Reiss, Carol.

In: Nature, Vol. 305, No. 5936, 1983, p. 726-729.

Research output: Contribution to journalArticle

Pober, JS, Collins, T, Gimbrone, MA, Cotran, RS, Gitlin, JD, Fiers, W, Clayberger, C, Krensky, AM, Burakoff, SJ & Reiss, C 1983, 'Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon', Nature, vol. 305, no. 5936, pp. 726-729. https://doi.org/10.1038/305726a0
Pober, Jordan S. ; Collins, Tucker ; Gimbrone, Michael A. ; Cotran, Ramzi S. ; Gitlin, Jonathan D. ; Fiers, Walter ; Clayberger, Carol ; Krensky, Alan M. ; Burakoff, Steven J. ; Reiss, Carol. / Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. In: Nature. 1983 ; Vol. 305, No. 5936. pp. 726-729.
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abstract = "T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1-3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B) 4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5-8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing 'passenger' leukocytes in rodent organ grafts appears necessary for graft rejection9-11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12-15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.",
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T1 - Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon

AU - Pober, Jordan S.

AU - Collins, Tucker

AU - Gimbrone, Michael A.

AU - Cotran, Ramzi S.

AU - Gitlin, Jonathan D.

AU - Fiers, Walter

AU - Clayberger, Carol

AU - Krensky, Alan M.

AU - Burakoff, Steven J.

AU - Reiss, Carol

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N2 - T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1-3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B) 4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5-8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing 'passenger' leukocytes in rodent organ grafts appears necessary for graft rejection9-11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12-15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

AB - T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1-3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B) 4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5-8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing 'passenger' leukocytes in rodent organ grafts appears necessary for graft rejection9-11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12-15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

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