Lymphocyte subpopulations in the neonate

High percentage of circulating B73.1+, HNK-1- cells

D. Montagna, P. Ferremi, Cristina Alberini, F. A. Porta, M. de Amici, G. C. Astaldi-Ricotti, R. Maccario, A. G. Ugazio

Research output: Contribution to journalArticle

Abstract

The reactivity of B73.1 monoclonal antibody was evaluated on unseparated and on T cell-depleted and T cell-enriched fractions of both cord blood lymphocytes (CBL) and adult peripheral blood lymphocytes (a-PBL). The reactivity of this monoclonal antibody with the E-, OKT3-, OKT8+, HNK-1- subset of CBL, previously demonstrated to have NK activity, was also studied. The data show that B73.1+ cells were present in similar percentage in CBL and a-PBL while HNK-1+ cells were very low in CBL. In addition the B73.1 monoclonal antibody reacted with about 50% of the E-, OKT3-, OKT8+, HNK-1- CBL and within this subset the majority of the B73.1+ cells were PNA-. These data support the hypothesis that part of this neonatal subpopulation, belongs to the NK cell lineage. Moreover the results obtained by double-labelling with B73.1 monoclonal antibody and PNA suggest that the E-, OKT3-, OKT8+, HNK-1- CBL include either different subsets or various differentiation stages of the same subset.

Original languageEnglish (US)
Pages (from-to)171-176
Number of pages6
JournalThymus
Volume8
Issue number3
StatePublished - 1986

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Lymphocyte Subsets
Fetal Blood
Lymphocytes
Muromonab-CD3
Monoclonal Antibodies
T-Lymphocytes
Cell Lineage
Natural Killer Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Montagna, D., Ferremi, P., Alberini, C., Porta, F. A., de Amici, M., Astaldi-Ricotti, G. C., ... Ugazio, A. G. (1986). Lymphocyte subpopulations in the neonate: High percentage of circulating B73.1+, HNK-1- cells. Thymus, 8(3), 171-176.

Lymphocyte subpopulations in the neonate : High percentage of circulating B73.1+, HNK-1- cells. / Montagna, D.; Ferremi, P.; Alberini, Cristina; Porta, F. A.; de Amici, M.; Astaldi-Ricotti, G. C.; Maccario, R.; Ugazio, A. G.

In: Thymus, Vol. 8, No. 3, 1986, p. 171-176.

Research output: Contribution to journalArticle

Montagna, D, Ferremi, P, Alberini, C, Porta, FA, de Amici, M, Astaldi-Ricotti, GC, Maccario, R & Ugazio, AG 1986, 'Lymphocyte subpopulations in the neonate: High percentage of circulating B73.1+, HNK-1- cells', Thymus, vol. 8, no. 3, pp. 171-176.
Montagna D, Ferremi P, Alberini C, Porta FA, de Amici M, Astaldi-Ricotti GC et al. Lymphocyte subpopulations in the neonate: High percentage of circulating B73.1+, HNK-1- cells. Thymus. 1986;8(3):171-176.
Montagna, D. ; Ferremi, P. ; Alberini, Cristina ; Porta, F. A. ; de Amici, M. ; Astaldi-Ricotti, G. C. ; Maccario, R. ; Ugazio, A. G. / Lymphocyte subpopulations in the neonate : High percentage of circulating B73.1+, HNK-1- cells. In: Thymus. 1986 ; Vol. 8, No. 3. pp. 171-176.
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AU - Porta, F. A.

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