Low-temperature plasma: An effective approach against Candida albicans biofilm

Juliana Aparecida Delben, Ramiro Mendonça Murata, Xi Wei, Myrella Lessio Castro, Wirley Gonçalves Assunção, Nelson Renato França Alves da Silva, Simone Duarte

Research output: Contribution to journalArticle

Abstract

This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.

Original languageEnglish (US)
Pages (from-to)231-244
Number of pages14
JournalPlasma Medicine
Volume4
Issue number1-4
DOIs
StatePublished - 2014

Fingerprint

biofilms
Candida
Biofilms
cold plasmas
Plasmas
Temperature
cells
Argon
proportion
argon
saliva
Plasma applications
perforation
Scanning electron microscopy
scanning electron microscopy
Assays
Microscopic examination
microscopy
Scanning

Keywords

  • Candida albicans
  • Low-temperature plasma
  • Oral biofilm

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physics and Astronomy(all)

Cite this

Delben, J. A., Murata, R. M., Wei, X., Castro, M. L., Assunção, W. G., da Silva, N. R. F. A., & Duarte, S. (2014). Low-temperature plasma: An effective approach against Candida albicans biofilm. Plasma Medicine, 4(1-4), 231-244. https://doi.org/10.1615/PlasmaMed.2014012361

Low-temperature plasma : An effective approach against Candida albicans biofilm. / Delben, Juliana Aparecida; Murata, Ramiro Mendonça; Wei, Xi; Castro, Myrella Lessio; Assunção, Wirley Gonçalves; da Silva, Nelson Renato França Alves; Duarte, Simone.

In: Plasma Medicine, Vol. 4, No. 1-4, 2014, p. 231-244.

Research output: Contribution to journalArticle

Delben, JA, Murata, RM, Wei, X, Castro, ML, Assunção, WG, da Silva, NRFA & Duarte, S 2014, 'Low-temperature plasma: An effective approach against Candida albicans biofilm', Plasma Medicine, vol. 4, no. 1-4, pp. 231-244. https://doi.org/10.1615/PlasmaMed.2014012361
Delben JA, Murata RM, Wei X, Castro ML, Assunção WG, da Silva NRFA et al. Low-temperature plasma: An effective approach against Candida albicans biofilm. Plasma Medicine. 2014;4(1-4):231-244. https://doi.org/10.1615/PlasmaMed.2014012361
Delben, Juliana Aparecida ; Murata, Ramiro Mendonça ; Wei, Xi ; Castro, Myrella Lessio ; Assunção, Wirley Gonçalves ; da Silva, Nelson Renato França Alves ; Duarte, Simone. / Low-temperature plasma : An effective approach against Candida albicans biofilm. In: Plasma Medicine. 2014 ; Vol. 4, No. 1-4. pp. 231-244.
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