Low-temperature plasma

An effective approach against Candida albicans biofilm

Juliana Aparecida Delben, Ramiro Mendonça Murata, Xi Wei, Myrella Lessio Castro, Wirley Gonçalves Assunção, Nelson Renato França Alves da Silva, Simone Duarte

Research output: Contribution to journalArticle

Abstract

This study evaluated the antifungal potential of low-temperature plasma (LTP) on a 72-hour Candida albicans biofilm. A growth inhibition zone test was conducted with agar plates inoculated with C. albicans and submitted to LTP and argon application at 3 and 10 mm for 10, 30, 60, 90, and 120 seconds. The groups for biofilm assays were 60 seconds of LTP application with a tip-to-sample distance of 3 mm (LTP-3) and 10 mm (LTP-10); –application of only argon gas for 60 seconds with a tip-to-sample distance of 3 mm (Ar-3) and 10 mm (Ar-10); and no treatment. The C. albicans biofilm was grown on saliva-coated discs. The medium was replaced every 24 hours. Confocal laser scanning microscopy revealed the proportion of live and dead cells, and variable pressure scanning electron microscopy (VPSEM) showed biofilm/cell structure. No inhibition zone was observed for control and either Ar groups. For the LTP groups, a progressively increasing of inhibition zone diameter was observed for different treatment durations. The LTP-3 and LTP-10 groups presented higher proportions of dead cells compared with the Ar-3 and Ar-10 groups. VPSEM revealed cell perforations in the LTP-3 and LTP-10 groups. A short period of LTP exposure demonstrated an antifungal effect on C. albicans biofilm.

Original languageEnglish (US)
Pages (from-to)231-244
Number of pages14
JournalPlasma Medicine
Volume4
Issue number1-4
DOIs
StatePublished - 2014

Fingerprint

biofilms
Candida
Biofilms
cold plasmas
Plasmas
Temperature
cells
Argon
proportion
argon
saliva
Plasma applications
perforation
Scanning electron microscopy
scanning electron microscopy
Assays
Microscopic examination
microscopy
Scanning

Keywords

  • Candida albicans
  • Low-temperature plasma
  • Oral biofilm

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physics and Astronomy(all)

Cite this

Delben, J. A., Murata, R. M., Wei, X., Castro, M. L., Assunção, W. G., da Silva, N. R. F. A., & Duarte, S. (2014). Low-temperature plasma: An effective approach against Candida albicans biofilm. Plasma Medicine, 4(1-4), 231-244. https://doi.org/10.1615/PlasmaMed.2014012361

Low-temperature plasma : An effective approach against Candida albicans biofilm. / Delben, Juliana Aparecida; Murata, Ramiro Mendonça; Wei, Xi; Castro, Myrella Lessio; Assunção, Wirley Gonçalves; da Silva, Nelson Renato França Alves; Duarte, Simone.

In: Plasma Medicine, Vol. 4, No. 1-4, 2014, p. 231-244.

Research output: Contribution to journalArticle

Delben, JA, Murata, RM, Wei, X, Castro, ML, Assunção, WG, da Silva, NRFA & Duarte, S 2014, 'Low-temperature plasma: An effective approach against Candida albicans biofilm', Plasma Medicine, vol. 4, no. 1-4, pp. 231-244. https://doi.org/10.1615/PlasmaMed.2014012361
Delben JA, Murata RM, Wei X, Castro ML, Assunção WG, da Silva NRFA et al. Low-temperature plasma: An effective approach against Candida albicans biofilm. Plasma Medicine. 2014;4(1-4):231-244. https://doi.org/10.1615/PlasmaMed.2014012361
Delben, Juliana Aparecida ; Murata, Ramiro Mendonça ; Wei, Xi ; Castro, Myrella Lessio ; Assunção, Wirley Gonçalves ; da Silva, Nelson Renato França Alves ; Duarte, Simone. / Low-temperature plasma : An effective approach against Candida albicans biofilm. In: Plasma Medicine. 2014 ; Vol. 4, No. 1-4. pp. 231-244.
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