Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice

Alesha Castillo, Imranul Alam, Shigeo M. Tanaka, Jeffery Levenda, Jiliang Li, Stuart J. Warden, Charles H. Turner

Research output: Contribution to journalArticle

Abstract

Mechanical loading of the skeleton is necessary to maintain bone structure and strength. Large amplitude strains associated with vigorous activity typically result in the greatest osteogenic response; however, data suggest that low-amplitude, broad-frequency vibration results in new bone formation and may enhance adaptation through a stochastic resonance (SR) phenomenon. That is, random noise may maximally enhance bone formation to a known osteogenic stimulus. The aims of this study were to (1) assess the ability of different vibration signals to enhance cortical bone formation during short- and long-term loading and (2) determine whether vibration could effect SR in bone. Two studies were completed wherein several osteogenic loading waveforms, with or without an additive low-amplitude, broad-frequency (0-50 Hz) vibration signal, were applied to the mouse ulna in axial compression. In study 1, mice were loaded short-term (30 s/day, 2 days) with either a carrier signal alone (1 or 2 N sine waveform), vibration signal alone [0.1 N or 0.3 N root mean square (RMS)] or combined carrier and vibration signal. In study 2, mice were loaded long-term (30 s/day, 3 days/week, 4 weeks) with a carrier signal alone (static or sine waveform), vibration signal alone (0.02 N, 0.04 N, 0.08 N or 0.25 N RMS) or combined carrier and vibration signal. Sequential calcein bone labels were administered at 2 and 4 days and at 4 and 29 days after the first day of loading in study 1 and 2, respectively; bone formation parameters and changes in geometry were measured. Combined application of the carrier and vibration signals in study 1 resulted in significantly greater bone formation than with either signal alone (P < 0.001); however, this increase was independently explained by increased strain levels associated with additive vibration. When load and strain levels were similar across loading groups in study 2, cortical bone formation and changes in geometry were not significantly altered by vibration. Vibration alone did not result in any new bone formation. Our data suggest that low-amplitude, broad-frequency vibration superimposed onto an osteogenic waveform or vibration alone does not enhance cortical bone adaptation at the frequencies, amplitudes and loading periods tested.

Original languageEnglish (US)
Pages (from-to)1087-1096
Number of pages10
JournalBone
Volume39
Issue number5
DOIs
StatePublished - Nov 2006

Fingerprint

Vibration
Osteogenesis
Cortical Bone
Bone and Bones
Ulna
Skeleton

Keywords

  • Biomechanics
  • Bone adaptation
  • Mechanical loading
  • Stochastic resonance
  • Vibration

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Castillo, A., Alam, I., Tanaka, S. M., Levenda, J., Li, J., Warden, S. J., & Turner, C. H. (2006). Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice. Bone, 39(5), 1087-1096. https://doi.org/10.1016/j.bone.2006.04.026

Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice. / Castillo, Alesha; Alam, Imranul; Tanaka, Shigeo M.; Levenda, Jeffery; Li, Jiliang; Warden, Stuart J.; Turner, Charles H.

In: Bone, Vol. 39, No. 5, 11.2006, p. 1087-1096.

Research output: Contribution to journalArticle

Castillo, A, Alam, I, Tanaka, SM, Levenda, J, Li, J, Warden, SJ & Turner, CH 2006, 'Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice', Bone, vol. 39, no. 5, pp. 1087-1096. https://doi.org/10.1016/j.bone.2006.04.026
Castillo, Alesha ; Alam, Imranul ; Tanaka, Shigeo M. ; Levenda, Jeffery ; Li, Jiliang ; Warden, Stuart J. ; Turner, Charles H. / Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice. In: Bone. 2006 ; Vol. 39, No. 5. pp. 1087-1096.
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abstract = "Mechanical loading of the skeleton is necessary to maintain bone structure and strength. Large amplitude strains associated with vigorous activity typically result in the greatest osteogenic response; however, data suggest that low-amplitude, broad-frequency vibration results in new bone formation and may enhance adaptation through a stochastic resonance (SR) phenomenon. That is, random noise may maximally enhance bone formation to a known osteogenic stimulus. The aims of this study were to (1) assess the ability of different vibration signals to enhance cortical bone formation during short- and long-term loading and (2) determine whether vibration could effect SR in bone. Two studies were completed wherein several osteogenic loading waveforms, with or without an additive low-amplitude, broad-frequency (0-50 Hz) vibration signal, were applied to the mouse ulna in axial compression. In study 1, mice were loaded short-term (30 s/day, 2 days) with either a carrier signal alone (1 or 2 N sine waveform), vibration signal alone [0.1 N or 0.3 N root mean square (RMS)] or combined carrier and vibration signal. In study 2, mice were loaded long-term (30 s/day, 3 days/week, 4 weeks) with a carrier signal alone (static or sine waveform), vibration signal alone (0.02 N, 0.04 N, 0.08 N or 0.25 N RMS) or combined carrier and vibration signal. Sequential calcein bone labels were administered at 2 and 4 days and at 4 and 29 days after the first day of loading in study 1 and 2, respectively; bone formation parameters and changes in geometry were measured. Combined application of the carrier and vibration signals in study 1 resulted in significantly greater bone formation than with either signal alone (P < 0.001); however, this increase was independently explained by increased strain levels associated with additive vibration. When load and strain levels were similar across loading groups in study 2, cortical bone formation and changes in geometry were not significantly altered by vibration. Vibration alone did not result in any new bone formation. Our data suggest that low-amplitude, broad-frequency vibration superimposed onto an osteogenic waveform or vibration alone does not enhance cortical bone adaptation at the frequencies, amplitudes and loading periods tested.",
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