Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip

Jesse V. Jokerst, Jie Chou, James P. Camp, Jorge Wong, Alexis Lennart, Amanda A. Pollard, Pierre N. Floriano, Nicolaos Christodoulides, Glennon W. Simmons, Yanjie Zhou, Mehnaaz F. Ali, John McDevitt

Research output: Contribution to journalArticle

Abstract

The slow development of cost-effective medical microdevices with strong analytical performance characteristics is due to a lack of selective and efficient analyte capture and signaling. The recently developed programmable bio-nano-chip (PBNC) is a flexible detection device with analytical behavior rivaling established macroscopic methods. The PBNC system employs ≈300 μm-diameter bead sensors composed of agarose "nanonets" that populate a microelectromechanical support structure with integrated microfluidic elements. The beads are an efficient and selective protein-capture medium suitable for the analysis of complex fluid samples. Microscopy and computational studies probe the 3D interior of the beads. The relative contributions that the capture and detection of moieties, analyte size, and bead porosity make to signal distribution and intensity are reported. Agarose pore sizes ranging from 45 to 620 nm are examined and those near 140 nm provide optimal transport characteristics for rapid (

Original languageEnglish (US)
Pages (from-to)613-624
Number of pages12
JournalSmall
Volume7
Issue number5
DOIs
StatePublished - Mar 7 2011

Fingerprint

Biomarkers
Microfluidics
Sepharose
Pore size
Microscopic examination
Porosity
Proteins
Fluids
Sensors
Costs
Microscopy
Costs and Cost Analysis
Equipment and Supplies

Keywords

  • agarose
  • biomarker capture
  • in vitro diagnostics
  • microfluidics
  • nanodevices
  • nanonet
  • programmable bio-nano-chips

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Jokerst, J. V., Chou, J., Camp, J. P., Wong, J., Lennart, A., Pollard, A. A., ... McDevitt, J. (2011). Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip. Small, 7(5), 613-624. https://doi.org/10.1002/smll.201002089

Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip. / Jokerst, Jesse V.; Chou, Jie; Camp, James P.; Wong, Jorge; Lennart, Alexis; Pollard, Amanda A.; Floriano, Pierre N.; Christodoulides, Nicolaos; Simmons, Glennon W.; Zhou, Yanjie; Ali, Mehnaaz F.; McDevitt, John.

In: Small, Vol. 7, No. 5, 07.03.2011, p. 613-624.

Research output: Contribution to journalArticle

Jokerst, JV, Chou, J, Camp, JP, Wong, J, Lennart, A, Pollard, AA, Floriano, PN, Christodoulides, N, Simmons, GW, Zhou, Y, Ali, MF & McDevitt, J 2011, 'Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip', Small, vol. 7, no. 5, pp. 613-624. https://doi.org/10.1002/smll.201002089
Jokerst JV, Chou J, Camp JP, Wong J, Lennart A, Pollard AA et al. Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip. Small. 2011 Mar 7;7(5):613-624. https://doi.org/10.1002/smll.201002089
Jokerst, Jesse V. ; Chou, Jie ; Camp, James P. ; Wong, Jorge ; Lennart, Alexis ; Pollard, Amanda A. ; Floriano, Pierre N. ; Christodoulides, Nicolaos ; Simmons, Glennon W. ; Zhou, Yanjie ; Ali, Mehnaaz F. ; McDevitt, John. / Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip. In: Small. 2011 ; Vol. 7, No. 5. pp. 613-624.
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