Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping

Bo Ram Oh, Nien Tsu Huang, Weiqiang Chen, Jungwhan Seo, Jianping Fu, Katsuo Kurabayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed an optofluidic biosensor by integrating a microfluidic device into a Localized Surface Plasmon Resonance (LSPR) detection system to quantitatively analyze a cell-secreted cytokine, TNF-alpha. The device was designed for on-chip trapping and incubation of immune cells and subsequent detection of cytokines by a gold nanoparticle-based LSPR sensor. During the 2 hour incubation of 50,000 THP-1 cells in the microfluidic device, we observed a shift of 0.2877nm in the LSPR surface absorbance spectrum peak, which corresponds to a TNF-alpha concentration of 327ng/mL. This device enables LSPR technique for cell-secreted cytokine assay detection by scaling down the cell secretion volume.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages92-94
Number of pages3
Volume1
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Surface plasmon resonance
Biosensors
Labels
Cytokines
Microfluidics
Tumor Necrosis Factor-alpha
Gold
Assays
Nanoparticles
Sensors

Keywords

  • Biosensor
  • Localized surface plasmon resonance (LSPR)
  • Optofluidic

ASJC Scopus subject areas

  • Bioengineering

Cite this

Oh, B. R., Huang, N. T., Chen, W., Seo, J., Fu, J., & Kurabayashi, K. (2013). Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 92-94). Chemical and Biological Microsystems Society.

Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping. / Oh, Bo Ram; Huang, Nien Tsu; Chen, Weiqiang; Seo, Jungwhan; Fu, Jianping; Kurabayashi, Katsuo.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 92-94.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Oh, BR, Huang, NT, Chen, W, Seo, J, Fu, J & Kurabayashi, K 2013, Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 92-94, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Oh BR, Huang NT, Chen W, Seo J, Fu J, Kurabayashi K. Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 92-94
Oh, Bo Ram ; Huang, Nien Tsu ; Chen, Weiqiang ; Seo, Jungwhan ; Fu, Jianping ; Kurabayashi, Katsuo. / Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 92-94
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