Localized maternal orthodenticle patterns anterior and posterior in the long germ wasp Nasonia

Jeremy A. Lynch, Ava E. Brent, David S. Leaf, Mary Anne Pultz, Claude Desplan

Research output: Contribution to journalArticle

Abstract

The Bicoid (Bcd) gradient in Drosophila has long been a model for the action of a morphogen in establishing embryonic polarity 1. However, it is now clear that bcd is a unique feature of higher Diptera2,3. An evolutionarily ancient gene, orthodenticle (otd), has a bcd-like role in the beetle Tribolium4. Unlike the Bcd gradient, which arises by diffusion of protein from an anteriorly localized messenger RNA1,5, the Tribolium Otd gradient forms by translational repression of otd mRNA by a posteriorly localized factor. These differences in gradient formation are correlated with differences in modes of embryonic patterning. Drosophila uses long germ embryogenesis, where the embryo derives from the entire anterior-posterior axis, and all segments are patterned at the blastoderm stage, before gastrulation. In contrast, Tribolium undergoes short germ embryogenesis: the embryo arises from cells in the posterior of the egg, and only anterior segments are patterned at the blastoderm stage, with the remaining segments arising after gastrulation from a growth zone. Here we describe the role of otd in the long germband embryo of the wasp Nasonia vitripennis. We show that Nasonia otd maternal mRNA is localized at both poles of the embryo, and resulting protein gradients pattern both poles. Thus, localized Nasonia otd has two major roles that allow long germ development. It activates anterior targets at the anterior of the egg in a manner reminiscent of the Bcd gradient, and it is required for pre-gastrulation expression of posterior gap genes.

Original languageEnglish (US)
Pages (from-to)728-732
Number of pages5
JournalNature
Volume439
Issue number7077
DOIs
StatePublished - Feb 9 2006

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Wasps
Gastrulation
Embryonic Structures
Mothers
Blastoderm
Tribolium
Drosophila
Embryonic Development
Ovum
Stored Messenger RNA
Beetles
Genes
Proteins
Messenger RNA
Growth

ASJC Scopus subject areas

  • General

Cite this

Localized maternal orthodenticle patterns anterior and posterior in the long germ wasp Nasonia. / Lynch, Jeremy A.; Brent, Ava E.; Leaf, David S.; Pultz, Mary Anne; Desplan, Claude.

In: Nature, Vol. 439, No. 7077, 09.02.2006, p. 728-732.

Research output: Contribution to journalArticle

Lynch, Jeremy A. ; Brent, Ava E. ; Leaf, David S. ; Pultz, Mary Anne ; Desplan, Claude. / Localized maternal orthodenticle patterns anterior and posterior in the long germ wasp Nasonia. In: Nature. 2006 ; Vol. 439, No. 7077. pp. 728-732.
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