Localization of 5-HT(1a) receptors to astroglial cells in adult rats

Implications for neuronal-glial interactions and psychoactive drug mechanism of action

P. M. Whitaker-Azmitia, C. Clarke, E. C. Azmitia

Research output: Contribution to journalArticle

Abstract

Although tissue culture studies have shown a variety of neurotransmitter receptors on astroglial cells, verifying these observations in adult animals has been difficult and rarely accomplished. In the current study we have used double immunocytochemistry to localize 5-HT(1a) receptors to astroglial cells in fixed sections of adult rat brain. The astroglial cells were identified using an antibody raised against the astroglial-specific protein glial fibrillary acidic protein (GFAP). To label the 5-HT(1a) receptor, we used an antibody we recently raised against a unique peptide sequence occurring in the second extracellular loop of the receptor. Our results show that the 5- HT(1a) receptor occurs in relatively high abundance on astroglial cells. There is regional specificity, the receptor being much more commonly found in septum and hippocampus than striatum. There are also intraregional differences in that even within a single brain region one astrocyte may have very high levels of the receptor while an adjacent cell has none. We propose that the cellular localization of this receptor could have significance in understanding the mechanism of action of 5-HT(1a) receptor active drugs in alleviating anxiety and depression. The mechanism may be through the release of a neurotrophic agent, S-100β, from astrocytes. This factor may then cause regeneration or sprouting of neuronal terminals which have been lost due to a disease process.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalSynapse
Volume14
Issue number3
StatePublished - 1993

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Receptor, Serotonin, 5-HT1A
Psychotropic Drugs
Neuroglia
Astrocytes
Neurotransmitter Receptor
Antibodies
Glial Fibrillary Acidic Protein
Brain
Regeneration
Hippocampus
Anxiety
Immunohistochemistry
Depression
Peptides
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)
  • Pharmacology

Cite this

Localization of 5-HT(1a) receptors to astroglial cells in adult rats : Implications for neuronal-glial interactions and psychoactive drug mechanism of action. / Whitaker-Azmitia, P. M.; Clarke, C.; Azmitia, E. C.

In: Synapse, Vol. 14, No. 3, 1993, p. 201-205.

Research output: Contribution to journalArticle

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