Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy

George Shubeita, S. K. Sekatskii, G. Dietler, V. S. Letokhov

Research output: Contribution to journalArticle

Abstract

We present fluorescence resonance energy transfer (FRET) images of donor dye molecule clusters recorded using a local fluorescence probe for scanning near-field optical microscopy (SNOM): standard apertured SNOM fiber tip coated with the 30-100-nm-thick polymer layer stained with the acceptor dye molecules. The tip works as a "self-sharpening pencil": the apical layers of the FRET-active tip coating are mechanically worn out during scanning thus continuously exposing a fresh active apex to continue imaging. Only a few tens of acceptor molecules are used to form the optical images, and using such an approach spatial resolution better than the size of the aperture is achievable.

Original languageEnglish (US)
Pages (from-to)2625-2627
Number of pages3
JournalApplied Physics Letters
Volume80
Issue number15
DOIs
StatePublished - Apr 15 2002

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resonance fluorescence
near fields
energy transfer
microscopy
scanning
probes
dyes
molecules
apexes
spatial resolution
apertures
coatings
fluorescence
fibers
polymers

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy. / Shubeita, George; Sekatskii, S. K.; Dietler, G.; Letokhov, V. S.

In: Applied Physics Letters, Vol. 80, No. 15, 15.04.2002, p. 2625-2627.

Research output: Contribution to journalArticle

Shubeita, George ; Sekatskii, S. K. ; Dietler, G. ; Letokhov, V. S. / Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy. In: Applied Physics Letters. 2002 ; Vol. 80, No. 15. pp. 2625-2627.
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