Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy

George Shubeita, S. K. Sekatskii, G. Dietler, V. S. Letokhov

    Research output: Contribution to journalArticle

    Abstract

    We present fluorescence resonance energy transfer (FRET) images of donor dye molecule clusters recorded using a local fluorescence probe for scanning near-field optical microscopy (SNOM): standard apertured SNOM fiber tip coated with the 30-100-nm-thick polymer layer stained with the acceptor dye molecules. The tip works as a "self-sharpening pencil": the apical layers of the FRET-active tip coating are mechanically worn out during scanning thus continuously exposing a fresh active apex to continue imaging. Only a few tens of acceptor molecules are used to form the optical images, and using such an approach spatial resolution better than the size of the aperture is achievable.

    Original languageEnglish (US)
    Pages (from-to)2625-2627
    Number of pages3
    JournalApplied Physics Letters
    Volume80
    Issue number15
    DOIs
    StatePublished - Apr 15 2002

    Fingerprint

    resonance fluorescence
    near fields
    energy transfer
    microscopy
    scanning
    probes
    dyes
    molecules
    apexes
    spatial resolution
    apertures
    coatings
    fluorescence
    fibers
    polymers

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

    Cite this

    Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy. / Shubeita, George; Sekatskii, S. K.; Dietler, G.; Letokhov, V. S.

    In: Applied Physics Letters, Vol. 80, No. 15, 15.04.2002, p. 2625-2627.

    Research output: Contribution to journalArticle

    Shubeita, George ; Sekatskii, S. K. ; Dietler, G. ; Letokhov, V. S. / Local fluorescent probes for the fluorescence resonance energy transfer scanning near-field optical microscopy. In: Applied Physics Letters. 2002 ; Vol. 80, No. 15. pp. 2625-2627.
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