Local and regional variations in myofibrillar patterns in looping rat hearts

R. L. Price, C. Chintanowonges, I. Shiraishi, T. K. Borg, Louis Terracio

Research output: Contribution to journalArticle

Abstract

Background: In chickens, cytodifferentiation, right side dominance in myofibril development, and variations in myofibrillar patterns in different areas and layers of the myocardial wall exist which have been implicated in the process of heart looping. Little comparable information is available for developing myofibrillar patterns in the early development of mammalian hearts. Methods: We have used transmission electron microscopy (TEM), confocal scanning laser microscopy (CSLM), and 3-D reconstruction techniques to determine if similar variations to those which exist in the chicken are also present in the looping hearts of embryonic day (ED) 9.5 to 11.5 rat hearts. Results: Local and regional variations and right side dominance in myofibrillar patterns were shown during looping in 9.5 through 11.5 days of development in embryonic rat heart. At 9.5 days of development, myofibrils near the lumen of the myocardial wall were primarily in circumferential bands while near the pericardial surface they were primarily in longitudinal bands. In older embryos, regional variations in myofibrillar organization was found in areas associated with the cardiac cushions, trabeculae, and myocardial wall of the developing heart chambers. Based on sarcomeric structure, myofibrils in the ventricle and outflow tract were more advanced than those found in the atrial wall. Conclusions: The local and regional patterns of myofibrils in looping rat hearts are similar to those which have been found in developing chicken hearts. This study and others indicate cytodifferentiation and development of the contractile apparatus has a crucial role in the process of heart looping.

Original languageEnglish (US)
Pages (from-to)83-93
Number of pages11
JournalAnatomical Record
Volume245
Issue number1
DOIs
StatePublished - May 1996

Fingerprint

heart
rats
Myofibrils
myofibrils
Chickens
regional pattern
chickens
transmission electron microscopy
microscopy
embryo
outflow
laser
confocal laser scanning microscopy
Transmission Electron Microscopy
Confocal Microscopy
Embryonic Development
early development
embryo (animal)
embryogenesis
Embryonic Structures

Keywords

  • Confocal Microscopy
  • Electron Microscopy
  • Heart Looping
  • Myofibril Pattern
  • Myofibrillogenesis
  • Rat

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Local and regional variations in myofibrillar patterns in looping rat hearts. / Price, R. L.; Chintanowonges, C.; Shiraishi, I.; Borg, T. K.; Terracio, Louis.

In: Anatomical Record, Vol. 245, No. 1, 05.1996, p. 83-93.

Research output: Contribution to journalArticle

Price, R. L. ; Chintanowonges, C. ; Shiraishi, I. ; Borg, T. K. ; Terracio, Louis. / Local and regional variations in myofibrillar patterns in looping rat hearts. In: Anatomical Record. 1996 ; Vol. 245, No. 1. pp. 83-93.
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