Abstract
Self-replication and exponential growth are ubiquitous in nature but until recently there were few examples of artificial self-replication. Often replication is a templated process where a parent produces a single offspring, doubling the population in each generation. Many species however produce more than one offspring at a time, enabling faster population growth and higher probability of species perpetuation. We have made a system of cross-shaped origami tiles that yields a number of offspring, four to eight or more, depending on the concentration of monomer units to be assembled. The parent dimer template serves as a seed to crystallize a one-dimensional crystal, a ladder. The ladder rungs are then UV-cross-linked and the offspring are then released by heating, to yield a litter of autonomous daughters. In the complement study, we also optimize the growth conditions to speed up the process and yield a 103 increase in the growth rate for the single-offspring replication system. Self-replication and exponential growth of autonomous motifs is useful for fundamental studies of selection and evolution as well as for materials design, fabrication, and directed evolution. Methods that increase the growth rate, the primary evolutionary drive, not only speed up experiments but provide additional mechanisms for evolving materials toward desired functionalities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1952-1957 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 116 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- 1D crystal
- Cross-tile DNA origami
- Exponential growth
- Multiple offspring
- Self-replication
ASJC Scopus subject areas
- General
Cite this
Litters of self-replicating origami cross-tiles. / Zhuo, Rebecca; Zhou, Feng; He, Xiaojin; Sha, Ruojie; Seeman, Nadrian; Chaikin, Paul M.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 6, 01.01.2019, p. 1952-1957.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Litters of self-replicating origami cross-tiles
AU - Zhuo, Rebecca
AU - Zhou, Feng
AU - He, Xiaojin
AU - Sha, Ruojie
AU - Seeman, Nadrian
AU - Chaikin, Paul M.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Self-replication and exponential growth are ubiquitous in nature but until recently there were few examples of artificial self-replication. Often replication is a templated process where a parent produces a single offspring, doubling the population in each generation. Many species however produce more than one offspring at a time, enabling faster population growth and higher probability of species perpetuation. We have made a system of cross-shaped origami tiles that yields a number of offspring, four to eight or more, depending on the concentration of monomer units to be assembled. The parent dimer template serves as a seed to crystallize a one-dimensional crystal, a ladder. The ladder rungs are then UV-cross-linked and the offspring are then released by heating, to yield a litter of autonomous daughters. In the complement study, we also optimize the growth conditions to speed up the process and yield a 103 increase in the growth rate for the single-offspring replication system. Self-replication and exponential growth of autonomous motifs is useful for fundamental studies of selection and evolution as well as for materials design, fabrication, and directed evolution. Methods that increase the growth rate, the primary evolutionary drive, not only speed up experiments but provide additional mechanisms for evolving materials toward desired functionalities.
AB - Self-replication and exponential growth are ubiquitous in nature but until recently there were few examples of artificial self-replication. Often replication is a templated process where a parent produces a single offspring, doubling the population in each generation. Many species however produce more than one offspring at a time, enabling faster population growth and higher probability of species perpetuation. We have made a system of cross-shaped origami tiles that yields a number of offspring, four to eight or more, depending on the concentration of monomer units to be assembled. The parent dimer template serves as a seed to crystallize a one-dimensional crystal, a ladder. The ladder rungs are then UV-cross-linked and the offspring are then released by heating, to yield a litter of autonomous daughters. In the complement study, we also optimize the growth conditions to speed up the process and yield a 103 increase in the growth rate for the single-offspring replication system. Self-replication and exponential growth of autonomous motifs is useful for fundamental studies of selection and evolution as well as for materials design, fabrication, and directed evolution. Methods that increase the growth rate, the primary evolutionary drive, not only speed up experiments but provide additional mechanisms for evolving materials toward desired functionalities.
KW - 1D crystal
KW - Cross-tile DNA origami
KW - Exponential growth
KW - Multiple offspring
KW - Self-replication
UR - http://www.scopus.com/inward/record.url?scp=85061121416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061121416&partnerID=8YFLogxK
U2 - 10.1073/pnas.1812793116
DO - 10.1073/pnas.1812793116
M3 - Article
C2 - 30674667
AN - SCOPUS:85061121416
VL - 116
SP - 1952
EP - 1957
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 6
ER -