Linear dichroism of electric field oriented bacteriochlorophyll a-protein from green photosynthetic bacteria

William B. Whitten, Robert M. Pearlstein, Edwin F. Phares, Nicholas Geacintov

Research output: Contribution to journalArticle

Abstract

Bacteriochlorophyll a-protein from Prosthecochloris aestuarii strain 2K was oriented in a pulsed electric field. The room temperature linear dichroism spectrum of the oriented protein in the Qy region of the bacteriochlorophyll a absorption exhibits a single asymmetrical peak at 813 nm with a shoulder extending to the blue. The ≈12 nm fullwidth of the linear dichroism peak is only about half that of the 300 K absorption spectrum. The linear dichroism at 813 nm was not saturated at field strengths of up to 15 kV/cm. The time dependence of the linear dichroism suggests that the orienting particles are aggregates of at least some tens of bacteriochlorophyll a-protein trimers. The linear dichroism peak coincides in wavelength with the 813-nm peak of the 300 K, 4th derivative absorption spectrum of the protein and is therefore attributed to the bacteriochlorophyll a Qy exciton transition observed in absorption at the same wavelength.

Original languageEnglish (US)
Pages (from-to)491-498
Number of pages8
JournalBBA - Bioenergetics
Volume503
Issue number3
DOIs
StatePublished - Sep 7 1978

Fingerprint

Bacteriochlorophylls
Dichroism
Bacteria
Electric fields
Absorption spectra
Proteins
Wavelength
Derivatives
Temperature

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

Linear dichroism of electric field oriented bacteriochlorophyll a-protein from green photosynthetic bacteria. / Whitten, William B.; Pearlstein, Robert M.; Phares, Edwin F.; Geacintov, Nicholas.

In: BBA - Bioenergetics, Vol. 503, No. 3, 07.09.1978, p. 491-498.

Research output: Contribution to journalArticle

Whitten, William B. ; Pearlstein, Robert M. ; Phares, Edwin F. ; Geacintov, Nicholas. / Linear dichroism of electric field oriented bacteriochlorophyll a-protein from green photosynthetic bacteria. In: BBA - Bioenergetics. 1978 ; Vol. 503, No. 3. pp. 491-498.
@article{7d8d9318d6ae4405adc75d5afea4f10c,
title = "Linear dichroism of electric field oriented bacteriochlorophyll a-protein from green photosynthetic bacteria",
abstract = "Bacteriochlorophyll a-protein from Prosthecochloris aestuarii strain 2K was oriented in a pulsed electric field. The room temperature linear dichroism spectrum of the oriented protein in the Qy region of the bacteriochlorophyll a absorption exhibits a single asymmetrical peak at 813 nm with a shoulder extending to the blue. The ≈12 nm fullwidth of the linear dichroism peak is only about half that of the 300 K absorption spectrum. The linear dichroism at 813 nm was not saturated at field strengths of up to 15 kV/cm. The time dependence of the linear dichroism suggests that the orienting particles are aggregates of at least some tens of bacteriochlorophyll a-protein trimers. The linear dichroism peak coincides in wavelength with the 813-nm peak of the 300 K, 4th derivative absorption spectrum of the protein and is therefore attributed to the bacteriochlorophyll a Qy exciton transition observed in absorption at the same wavelength.",
author = "Whitten, {William B.} and Pearlstein, {Robert M.} and Phares, {Edwin F.} and Nicholas Geacintov",
year = "1978",
month = "9",
day = "7",
doi = "10.1016/0005-2728(78)90148-2",
language = "English (US)",
volume = "503",
pages = "491--498",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Linear dichroism of electric field oriented bacteriochlorophyll a-protein from green photosynthetic bacteria

AU - Whitten, William B.

AU - Pearlstein, Robert M.

AU - Phares, Edwin F.

AU - Geacintov, Nicholas

PY - 1978/9/7

Y1 - 1978/9/7

N2 - Bacteriochlorophyll a-protein from Prosthecochloris aestuarii strain 2K was oriented in a pulsed electric field. The room temperature linear dichroism spectrum of the oriented protein in the Qy region of the bacteriochlorophyll a absorption exhibits a single asymmetrical peak at 813 nm with a shoulder extending to the blue. The ≈12 nm fullwidth of the linear dichroism peak is only about half that of the 300 K absorption spectrum. The linear dichroism at 813 nm was not saturated at field strengths of up to 15 kV/cm. The time dependence of the linear dichroism suggests that the orienting particles are aggregates of at least some tens of bacteriochlorophyll a-protein trimers. The linear dichroism peak coincides in wavelength with the 813-nm peak of the 300 K, 4th derivative absorption spectrum of the protein and is therefore attributed to the bacteriochlorophyll a Qy exciton transition observed in absorption at the same wavelength.

AB - Bacteriochlorophyll a-protein from Prosthecochloris aestuarii strain 2K was oriented in a pulsed electric field. The room temperature linear dichroism spectrum of the oriented protein in the Qy region of the bacteriochlorophyll a absorption exhibits a single asymmetrical peak at 813 nm with a shoulder extending to the blue. The ≈12 nm fullwidth of the linear dichroism peak is only about half that of the 300 K absorption spectrum. The linear dichroism at 813 nm was not saturated at field strengths of up to 15 kV/cm. The time dependence of the linear dichroism suggests that the orienting particles are aggregates of at least some tens of bacteriochlorophyll a-protein trimers. The linear dichroism peak coincides in wavelength with the 813-nm peak of the 300 K, 4th derivative absorption spectrum of the protein and is therefore attributed to the bacteriochlorophyll a Qy exciton transition observed in absorption at the same wavelength.

UR - http://www.scopus.com/inward/record.url?scp=0018127276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018127276&partnerID=8YFLogxK

U2 - 10.1016/0005-2728(78)90148-2

DO - 10.1016/0005-2728(78)90148-2

M3 - Article

C2 - 687613

AN - SCOPUS:0018127276

VL - 503

SP - 491

EP - 498

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 3

ER -