Light-mediated inhibition of protein synthesis

Michael Goard, Girish Aakalu, Olesya D. Fedoryak, Carlo Quinonez, Jamii St. Julien, Stephen J. Poteet, Erin M. Schuman, Timothy Dore

    Research output: Contribution to journalArticle

    Abstract

    The regulation of protein synthesis is vital for a host of cell biological processes, but investigating roles for protein synthesis have been hindered by the inability to selectively interfere with it. To inhibit protein synthesis with spatial and temporal control, we have developed a photo-releasable anisomycin compound, N-([6-bromo-7-hydroxycoumarin-4-yl]methyloxycarbonyl) anisomycin (Bhc-Aniso), that can be removed through exposure to UV light. The area of protein synthesis inhibition can be restricted to a small light-exposed region or, potentially, the volume of two-photon excitation if a pulsed IR laser is the light source. We have tested the compound's effectiveness with an in vitro protein-translation system, CHO cells, HEK293 cells, and neurons. The photo-released anisomycin can inhibit protein synthesis in a spatially restricted manner, which will enable the specific inhibition of protein synthesis in subsets of cells with temporal and spatial precision.

    Original languageEnglish (US)
    Pages (from-to)685-693
    Number of pages9
    JournalChemistry and Biology
    Volume12
    Issue number6
    DOIs
    StatePublished - Jan 1 2005

    Fingerprint

    Anisomycin
    Light
    Proteins
    7-hydroxycoumarin
    Biological Phenomena
    CHO Cells
    HEK293 Cells
    Protein Biosynthesis
    Ultraviolet Rays
    Photons
    Ultraviolet radiation
    Lasers
    Neurons
    Light sources

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Pharmacology
    • Drug Discovery
    • Clinical Biochemistry

    Cite this

    Goard, M., Aakalu, G., Fedoryak, O. D., Quinonez, C., St. Julien, J., Poteet, S. J., ... Dore, T. (2005). Light-mediated inhibition of protein synthesis. Chemistry and Biology, 12(6), 685-693. https://doi.org/10.1016/j.chembiol.2005.04.018

    Light-mediated inhibition of protein synthesis. / Goard, Michael; Aakalu, Girish; Fedoryak, Olesya D.; Quinonez, Carlo; St. Julien, Jamii; Poteet, Stephen J.; Schuman, Erin M.; Dore, Timothy.

    In: Chemistry and Biology, Vol. 12, No. 6, 01.01.2005, p. 685-693.

    Research output: Contribution to journalArticle

    Goard, M, Aakalu, G, Fedoryak, OD, Quinonez, C, St. Julien, J, Poteet, SJ, Schuman, EM & Dore, T 2005, 'Light-mediated inhibition of protein synthesis', Chemistry and Biology, vol. 12, no. 6, pp. 685-693. https://doi.org/10.1016/j.chembiol.2005.04.018
    Goard M, Aakalu G, Fedoryak OD, Quinonez C, St. Julien J, Poteet SJ et al. Light-mediated inhibition of protein synthesis. Chemistry and Biology. 2005 Jan 1;12(6):685-693. https://doi.org/10.1016/j.chembiol.2005.04.018
    Goard, Michael ; Aakalu, Girish ; Fedoryak, Olesya D. ; Quinonez, Carlo ; St. Julien, Jamii ; Poteet, Stephen J. ; Schuman, Erin M. ; Dore, Timothy. / Light-mediated inhibition of protein synthesis. In: Chemistry and Biology. 2005 ; Vol. 12, No. 6. pp. 685-693.
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