Light-induced transcriptional repression of the pea AS1 gene

Identification of cis-elements and transfactors

Nora Ngai, Fong Ying Tsai, Gloria Coruzzi

Research output: Contribution to journalArticle

Abstract

Here, we examine the cis-elements and trans-factors affecting the expression of asparagine synthetase (AS) genes whose transcription is negatively regulated by light. The promoters for the AS1 and AS2 genes of pea were isolated, sequenced, and functionally dissected for their ability to confer regulated expression to the GUS reporter gene in transgenic tobacco. Histochemical analysis of transgenic plants demonstrated that the AS1 and AS2 promoters show identical patterns of cell-specific expression. The more highly active AS1 promoter was further demonstrated to confer negative light-regulation to the GUS gene in transgenic tobacco. Deletion analysis and gain-of-function experiments showed that 124 bp of the AS1 promoter was sufficient to confer light-activated repression to a heterologous promoter. Potential conserved transcription regulatory elements, Box B, Box C, and Box C' within this region were shown to bind to nuclear proteins by gel shift analysis. A light-specific DNA:protein interaction was detected with Box B. The nuclear factors that bind to Box C and C' elements of AS1 are competed by a putative repressor element 'RE1' defined previously in the oat phytochrome gene whose transcription is also repressed by light. The Box B and C/C'-Box/RE1-binding factors were found in nuclear extracts of tobacco, pea, and Arabidopsis and may therefore be universal factors involved in light-activated transcriptional repression.

Original languageEnglish (US)
Pages (from-to)1021-1034
Number of pages14
JournalPlant Journal
Volume12
Issue number5
StatePublished - Nov 1997

Fingerprint

Peas
peas
Light
promoter regions
Genes
Tobacco
tobacco
genes
transcription (genetics)
Aspartate-Ammonia Ligase
aspartate-ammonia ligase
genetically modified organisms
Phytochrome
Genetically Modified Plants
nuclear proteins
Electrophoretic Mobility Shift Assay
phytochrome
Nuclear Proteins
Reporter Genes
Arabidopsis

ASJC Scopus subject areas

  • Plant Science

Cite this

Light-induced transcriptional repression of the pea AS1 gene : Identification of cis-elements and transfactors. / Ngai, Nora; Tsai, Fong Ying; Coruzzi, Gloria.

In: Plant Journal, Vol. 12, No. 5, 11.1997, p. 1021-1034.

Research output: Contribution to journalArticle

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