Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles

Patrick Urban, Stefanie D. Pritzl, David B. Konrad, James A. Frank, Carla Pernpeintner, Christian R. Roeske, Dirk Trauner, Theobald Lohmüller

Research output: Contribution to journalArticle

Abstract

Controlling lateral interactions between lipid molecules in a bilayer membrane to guide membrane organization and domain formation is a key factor for studying and emulating membrane functionality in synthetic biological systems. Here, we demonstrate an approach to reversibly control lipid organization, domain formation, and membrane stiffness of phospholipid bilayer membranes using the photoswitchable phospholipid azo-PC. azo-PC contains an azobenzene group in the sn2 acyl chain that undergoes reversible photoisomerization on illumination with UV-A and visible light. We demonstrate that the concentration of the photolipid molecules and also the assembly and disassembly of photolipids into lipid domains can be monitored by UV-vis spectroscopy because of a blue shift induced by photolipid aggregation.

Original languageEnglish (US)
Pages (from-to)13368-13374
Number of pages7
JournalLangmuir
Volume34
Issue number44
DOIs
StatePublished - Nov 6 2018

Fingerprint

Lipids
lipids
membranes
Membranes
Phospholipids
interactions
Photoisomerization
Molecules
Azobenzene
Biological systems
Ultraviolet spectroscopy
blue shift
molecules
stiffness
Agglomeration
assembly
Lighting
illumination
Stiffness
spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Urban, P., Pritzl, S. D., Konrad, D. B., Frank, J. A., Pernpeintner, C., Roeske, C. R., ... Lohmüller, T. (2018). Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles. Langmuir, 34(44), 13368-13374. https://doi.org/10.1021/acs.langmuir.8b03241

Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles. / Urban, Patrick; Pritzl, Stefanie D.; Konrad, David B.; Frank, James A.; Pernpeintner, Carla; Roeske, Christian R.; Trauner, Dirk; Lohmüller, Theobald.

In: Langmuir, Vol. 34, No. 44, 06.11.2018, p. 13368-13374.

Research output: Contribution to journalArticle

Urban, P, Pritzl, SD, Konrad, DB, Frank, JA, Pernpeintner, C, Roeske, CR, Trauner, D & Lohmüller, T 2018, 'Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles', Langmuir, vol. 34, no. 44, pp. 13368-13374. https://doi.org/10.1021/acs.langmuir.8b03241
Urban P, Pritzl SD, Konrad DB, Frank JA, Pernpeintner C, Roeske CR et al. Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles. Langmuir. 2018 Nov 6;34(44):13368-13374. https://doi.org/10.1021/acs.langmuir.8b03241
Urban, Patrick ; Pritzl, Stefanie D. ; Konrad, David B. ; Frank, James A. ; Pernpeintner, Carla ; Roeske, Christian R. ; Trauner, Dirk ; Lohmüller, Theobald. / Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles. In: Langmuir. 2018 ; Vol. 34, No. 44. pp. 13368-13374.
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