Light-activated serotonin for exploring its action in biological systems

Adam C. Rea, Laura N. Vandenberg, Rebecca E. Ball, Ashley A. Snouffer, Alicia G. Hudson, Yue Zhu, Duncan E. McLain, Lindsey L. Johnston, James D. Lauderdale, Michael Levin, Timothy Dore

Research output: Contribution to journalArticle

Abstract

Summary Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.

Original languageEnglish (US)
Pages (from-to)1536-1546
Number of pages11
JournalChemistry and Biology
Volume20
Issue number12
DOIs
StatePublished - Dec 19 2013

Fingerprint

Biological systems
Serotonin
Light
Photons
Physiological Phenomena
Trigeminal Ganglion
Defects
Superior Colliculi
Physiology
Xenopus laevis
Appetite
Zebrafish
Sensory Receptor Cells
Neurons
Embryonic Development
Larva
Neurotransmitter Agents
Optics
Embryonic Structures
Learning

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Rea, A. C., Vandenberg, L. N., Ball, R. E., Snouffer, A. A., Hudson, A. G., Zhu, Y., ... Dore, T. (2013). Light-activated serotonin for exploring its action in biological systems. Chemistry and Biology, 20(12), 1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005

Light-activated serotonin for exploring its action in biological systems. / Rea, Adam C.; Vandenberg, Laura N.; Ball, Rebecca E.; Snouffer, Ashley A.; Hudson, Alicia G.; Zhu, Yue; McLain, Duncan E.; Johnston, Lindsey L.; Lauderdale, James D.; Levin, Michael; Dore, Timothy.

In: Chemistry and Biology, Vol. 20, No. 12, 19.12.2013, p. 1536-1546.

Research output: Contribution to journalArticle

Rea, AC, Vandenberg, LN, Ball, RE, Snouffer, AA, Hudson, AG, Zhu, Y, McLain, DE, Johnston, LL, Lauderdale, JD, Levin, M & Dore, T 2013, 'Light-activated serotonin for exploring its action in biological systems', Chemistry and Biology, vol. 20, no. 12, pp. 1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005
Rea AC, Vandenberg LN, Ball RE, Snouffer AA, Hudson AG, Zhu Y et al. Light-activated serotonin for exploring its action in biological systems. Chemistry and Biology. 2013 Dec 19;20(12):1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005
Rea, Adam C. ; Vandenberg, Laura N. ; Ball, Rebecca E. ; Snouffer, Ashley A. ; Hudson, Alicia G. ; Zhu, Yue ; McLain, Duncan E. ; Johnston, Lindsey L. ; Lauderdale, James D. ; Levin, Michael ; Dore, Timothy. / Light-activated serotonin for exploring its action in biological systems. In: Chemistry and Biology. 2013 ; Vol. 20, No. 12. pp. 1536-1546.
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