Light-activated serotonin for exploring its action in biological systems

Adam C. Rea, Laura N. Vandenberg, Rebecca E. Ball, Ashley A. Snouffer, Alicia G. Hudson, Yue Zhu, Duncan E. McLain, Lindsey L. Johnston, James D. Lauderdale, Michael Levin, Timothy Dore

    Research output: Contribution to journalArticle

    Abstract

    Summary Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.

    Original languageEnglish (US)
    Pages (from-to)1536-1546
    Number of pages11
    JournalChemistry and Biology
    Volume20
    Issue number12
    DOIs
    StatePublished - Dec 19 2013

    Fingerprint

    Biological systems
    Serotonin
    Light
    Photons
    Physiological Phenomena
    Trigeminal Ganglion
    Defects
    Superior Colliculi
    Physiology
    Xenopus laevis
    Appetite
    Zebrafish
    Sensory Receptor Cells
    Neurons
    Embryonic Development
    Larva
    Neurotransmitter Agents
    Optics
    Embryonic Structures
    Learning

    ASJC Scopus subject areas

    • Medicine(all)
    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Pharmacology
    • Drug Discovery
    • Clinical Biochemistry

    Cite this

    Rea, A. C., Vandenberg, L. N., Ball, R. E., Snouffer, A. A., Hudson, A. G., Zhu, Y., ... Dore, T. (2013). Light-activated serotonin for exploring its action in biological systems. Chemistry and Biology, 20(12), 1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005

    Light-activated serotonin for exploring its action in biological systems. / Rea, Adam C.; Vandenberg, Laura N.; Ball, Rebecca E.; Snouffer, Ashley A.; Hudson, Alicia G.; Zhu, Yue; McLain, Duncan E.; Johnston, Lindsey L.; Lauderdale, James D.; Levin, Michael; Dore, Timothy.

    In: Chemistry and Biology, Vol. 20, No. 12, 19.12.2013, p. 1536-1546.

    Research output: Contribution to journalArticle

    Rea, AC, Vandenberg, LN, Ball, RE, Snouffer, AA, Hudson, AG, Zhu, Y, McLain, DE, Johnston, LL, Lauderdale, JD, Levin, M & Dore, T 2013, 'Light-activated serotonin for exploring its action in biological systems', Chemistry and Biology, vol. 20, no. 12, pp. 1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005
    Rea AC, Vandenberg LN, Ball RE, Snouffer AA, Hudson AG, Zhu Y et al. Light-activated serotonin for exploring its action in biological systems. Chemistry and Biology. 2013 Dec 19;20(12):1536-1546. https://doi.org/10.1016/j.chembiol.2013.11.005
    Rea, Adam C. ; Vandenberg, Laura N. ; Ball, Rebecca E. ; Snouffer, Ashley A. ; Hudson, Alicia G. ; Zhu, Yue ; McLain, Duncan E. ; Johnston, Lindsey L. ; Lauderdale, James D. ; Levin, Michael ; Dore, Timothy. / Light-activated serotonin for exploring its action in biological systems. In: Chemistry and Biology. 2013 ; Vol. 20, No. 12. pp. 1536-1546.
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