Light-activated ion channels for remote control of neuronal firing

Matthew Banghart, Katharine Borges, Ehud Isacoff, Dirk Trauner, Richard H. Kramer

Research output: Contribution to journalArticle

Abstract

Neurons have ion channels that are directly gated by voltage, ligands and temperature but not by light. Using structure-based design, we have developed a new chemical gate that confers light sensitivity to an ion channel. The gate includes a functional group for selective conjugation to an engineered K + channel, a pore blocker and a photoisomerizable azobenzene. Long-wavelength light drives the azobenzene moiety into its extended trans configuration, allowing the blocker to reach the pore. Short-wavelength light generates the shorter cis configuration, retracting the blocker and allowing conduction. Exogenous expression of these channels in rat hippocampal neurons, followed by chemical modification with the photoswitchable gate, enables different wavelengths of light to switch action potential firing on and off. These synthetic photoisomerizable azobenzene-regulated K+ (SPARK) channels allow rapid, precise and reversible control over neuronal firing, with potential applications for dissecting neural circuits and controlling activity downstream from sites of neural damage or degeneration.

Original languageEnglish (US)
Pages (from-to)1381-1386
Number of pages6
JournalNature Neuroscience
Volume7
Issue number12
DOIs
StatePublished - Dec 2004

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Ion Channels
Light
Neurons
Photophobia
Action Potentials
Ligands
Temperature
azobenzene

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Banghart, M., Borges, K., Isacoff, E., Trauner, D., & Kramer, R. H. (2004). Light-activated ion channels for remote control of neuronal firing. Nature Neuroscience, 7(12), 1381-1386. https://doi.org/10.1038/nn1356

Light-activated ion channels for remote control of neuronal firing. / Banghart, Matthew; Borges, Katharine; Isacoff, Ehud; Trauner, Dirk; Kramer, Richard H.

In: Nature Neuroscience, Vol. 7, No. 12, 12.2004, p. 1381-1386.

Research output: Contribution to journalArticle

Banghart, M, Borges, K, Isacoff, E, Trauner, D & Kramer, RH 2004, 'Light-activated ion channels for remote control of neuronal firing', Nature Neuroscience, vol. 7, no. 12, pp. 1381-1386. https://doi.org/10.1038/nn1356
Banghart M, Borges K, Isacoff E, Trauner D, Kramer RH. Light-activated ion channels for remote control of neuronal firing. Nature Neuroscience. 2004 Dec;7(12):1381-1386. https://doi.org/10.1038/nn1356
Banghart, Matthew ; Borges, Katharine ; Isacoff, Ehud ; Trauner, Dirk ; Kramer, Richard H. / Light-activated ion channels for remote control of neuronal firing. In: Nature Neuroscience. 2004 ; Vol. 7, No. 12. pp. 1381-1386.
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