Lectin microarrays for glycoprotein analysis.

Kanoelani T. Pilobello, Lara Mahal

Research output: Contribution to journalArticle

Abstract

Glycosylation is one of the most common posttranslational modifications, with more than half of all known proteins thought to be glycoproteins. Alterations in glycosylation play a role in a diverse set of biological phenomena including tumor cell metastasis, intracellular communication, and inflammation. The complexity of glycosylation at the molecular level and the lack of rapid analytical tools complicate the study of glycan function. We have recently developed a lectin microarray for the high-throughput analysis of glycosylation. Lectins are carbohydrate-binding proteins that have been used for decades as a detection method for glycans. By placing the lectins in a microarray format and using standard microarray printing and scanning technology, we have created a simple yet powerful technique for glycan profiling.

Original languageEnglish (US)
Pages (from-to)193-203
Number of pages11
JournalMethods in Molecular Biology
Volume385
StatePublished - 2007

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Microarray Analysis
Glycosylation
Lectins
Glycoproteins
Polysaccharides
Biological Phenomena
Printing
Post Translational Protein Processing
Neoplasm Metastasis
Inflammation
Technology
Neoplasms
Proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Lectin microarrays for glycoprotein analysis. / Pilobello, Kanoelani T.; Mahal, Lara.

In: Methods in Molecular Biology, Vol. 385, 2007, p. 193-203.

Research output: Contribution to journalArticle

Pilobello, Kanoelani T. ; Mahal, Lara. / Lectin microarrays for glycoprotein analysis. In: Methods in Molecular Biology. 2007 ; Vol. 385. pp. 193-203.
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