Layer I Interneurons Sharpen Sensory Maps during Neonatal Development

Alicia Che, Rachel Babij, Andrew F. Iannone, Robert N. Fetcho, Monica Ferrer, Conor Liston, Gordon Fishell, Natalia V. De Marco García

Research output: Contribution to journalArticle

Abstract

The neonatal mammal faces an array of sensory stimuli when diverse neuronal types have yet to form sensory maps. How these inputs interact with intrinsic neuronal activity to facilitate circuit assembly is not well understood. By using longitudinal calcium imaging in unanesthetized mouse pups, we show that layer I (LI) interneurons, delineated by co-expression of the 5HT3a serotonin receptor (5HT3aR) and reelin (Re), display spontaneous calcium transients with the highest degree of synchrony among cell types present in the superficial barrel cortex at postnatal day 6 (P6). 5HT3aR Re interneurons are activated by whisker stimulation during this period, and sensory deprivation induces decorrelation of their activity. Moreover, attenuation of thalamic inputs through knockdown of NMDA receptors (NMDARs) in these interneurons results in expansion of whisker responses, aberrant barrel map formation, and deficits in whisker-dependent behavior. These results indicate that recruitment of specific interneuron types during development is critical for adult somatosensory function. Che et al. combine genetic tools and longitudinal in vivo calcium imaging in young mouse pups to show that superficial interneurons participate in a transient thalamocortical circuit. Their recruitment is critical for the emergence of sensory maps and sensory discrimination.

Original languageEnglish (US)
JournalNeuron
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Interneurons
Vibrissae
Serotonin Receptors
Calcium
Sensory Deprivation
N-Methyl-D-Aspartate Receptors
Mammals

Keywords

  • barrel cortex
  • calcium imaging
  • development
  • interneuron
  • layer I
  • spontaneous activity
  • thalamocortical connectivity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Che, A., Babij, R., Iannone, A. F., Fetcho, R. N., Ferrer, M., Liston, C., ... De Marco García, N. V. (Accepted/In press). Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. Neuron. https://doi.org/10.1016/j.neuron.2018.06.002

Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. / Che, Alicia; Babij, Rachel; Iannone, Andrew F.; Fetcho, Robert N.; Ferrer, Monica; Liston, Conor; Fishell, Gordon; De Marco García, Natalia V.

In: Neuron, 01.01.2018.

Research output: Contribution to journalArticle

Che, A, Babij, R, Iannone, AF, Fetcho, RN, Ferrer, M, Liston, C, Fishell, G & De Marco García, NV 2018, 'Layer I Interneurons Sharpen Sensory Maps during Neonatal Development', Neuron. https://doi.org/10.1016/j.neuron.2018.06.002
Che A, Babij R, Iannone AF, Fetcho RN, Ferrer M, Liston C et al. Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. Neuron. 2018 Jan 1. https://doi.org/10.1016/j.neuron.2018.06.002
Che, Alicia ; Babij, Rachel ; Iannone, Andrew F. ; Fetcho, Robert N. ; Ferrer, Monica ; Liston, Conor ; Fishell, Gordon ; De Marco García, Natalia V. / Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. In: Neuron. 2018.
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